二甲双胍对多囊卵巢综合征大鼠血清中网膜素-1水平的影响-东南大学学报医学版

二甲双胍对多囊卵巢综合征大鼠血清中网膜素-1水平的影响

单位：河南省中医院生殖医学科, 河南郑州 450002

分类号：R711.5

出版年·卷·期（页码）：2019·38·第一期（23-27）

摘要：

目的：探讨二甲双胍对多囊卵巢综合征大鼠血清中网膜素-1水平的影响。方法：将36只大鼠分为模型组、二甲双胍组、对照组，HE染色观察卵巢组织病理学变化，测定空腹血糖（FPG）、1 hPG、2 hPG、糖耐量AUC及血清睾酮（T）、孕激素（P）、雌二醇（E2）、卵泡刺激素（FSH）、黄体生成素（LH）、空腹胰岛素（FINS）、网膜素-1水平。结果：模型组大鼠卵巢组织卵泡膜细胞层增厚，颗粒细胞层数减少，黄体组织少，对照组大鼠卵巢组织中颗粒细胞呈多层，见多个黄体；二甲双胍组卵巢组织病理学改变介于模型组和对照组之间；模型组大鼠卵巢指数、血清T、LH、FPG、1hPG、2hPG、FINS、稳态模型胰岛素抵抗（HOMA-IR）水平均高于对照组，血清网膜素-1、血清P、E2、FSH水平均低于对照组（P<0.05）；二甲双胍组大鼠卵巢指数、血清T、FPG、1hPG、2hPG、FINS、HOMA-IR水平均低于模型组，血清网膜素-1、血清P、E2水平均高于模型组（P<0.05）；血清网膜素-1与血清P呈正相关（P<0.05），与T、FINS、HOMA-IR呈负相关（P<0.05）。结论：二甲双弧能显著的改善多囊卵巢综合症，为临床提供了实验依据。

Objective:To investigate the effect of metformin on the level of omentin-1 in serum of rats with polycystic ovary syndrome (PCOS). Methods:Thirty-six rats were divided into three groups:model group, metformin group and control group. HE staining to observe the histopathological changes of ovary, fasting blood glucose (FPG), 1hPG,2hPG, glucose tolerance AUC and serum testosterone (T), progesterone (P), Estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH), fasting insulin (FINS), omentin-1 level. Results:In the model group, the follicular membrane cell layer was thickened, the number of granulosa cell layer was decreased, and the luteal tissue was less. In the control group, the granulosa cells in the ovarian tissue were multilayered and multiple luteal bodies were found. The histopathological changes of ovary in metformin group were in between the model group and the control group. The ovarian index, 1hPG, 2hPG and HOMA-IR levels in the model group were significantly higher than those in the control group, and the levels of omentin-1 and serum P,E2,FSH in the model group were significantly lower than those in the control group (P<0.05), and the level of insulin resistance (HOMA-IR) in the steady-state model of FINS was significantly lower than that in the control group. The difference was statistically significant (P<0.05). In metformin group, the ovarian index, serum T,FPG,1hPG,2hPG and FINS,HOMA-IR levels were lower than those in the model group, and the levels of omentin-1 and serum P,T,E2 were significantly higher than those in the model group (P<0.05). Serum omentin-1 was positively correlated with serum P (P<0.05), and negatively correlated with T,FINS,HOMA-IR (P<0.05). Conclusion:Metformin can improve the polycystic ovary syndrome significantly, which provides experimental basis for clinical practice.

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