Objective:To investigate the effect of parecoxib on postoperative cognitive dysfunction of geriatric rats and the possible mechanism. Methods:One hundred geriatric SD rats were randomly divided into control group, model group, treatment group. Abbreviated laparotomy was used by rats in the model group and the treatment group to build postoperative cognitive dysfunction (POCD) model. The rats in the treatment group were administrated 5 mg·kg-1 parecoxib and rats in the model group were administrated saline (the same volume as the treatment group). Morris water maze was performed to evaluate the learning cognition of rats. Immunohistochemistry was performed to determine the activation of hippocampal astrocytes. Enzyme-linked immunosorbent assay (ELISA) was used to determine the expression of IL-1β, IL-6 and TNF-α in the hippocampus of rats. Western blot was used to determine the expression of COX-2 and iNOS in the hippocampal of rats. Results:Compared with the model group, the avoidance latency of Morris water maze in the treatment group was significantly decreased while the number of crossings was significantly increased (P<0.05). Compared with the model group, average optical density of astrocyte, IL-1β, IL-6, TNF-α, COX-2, iNOS in hippocampusin were significantly decreased in the hippocampus region of the treatment group (P<0.05). Conclusion:Paracoxib can protect the postoperative cognitive dysfunction of geriatric rats by inhibiting of COX-2, decreasing expression of IL-1β, IL-6, TNF-α in the hippocampus, reducing the expression of iNOS.
 LI F,WANG Y,YU L,et al.Viral infection of the central nervous system and neuroin flammation precede blood-brain barrier disruption during Japanese encephalitis virus infection[J].J Virol,2015,89(10):5602-5614.
 BLANCO A M,PASCUAL M,VALLES S L,et al.Ethanol-induced iNOS and COX-2 expression in cultured astrocytes via NF-kappa B[J].Neuroreport,2004,15(4):681-685.
 YONG H C,KIM H J,DONG I K,et al.Effect of garlic (Allium sativum L.) stems on inflammatory cytokines,iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide[J].Comunicar,2015,22(4):613-621.