Objective:To investigate the relationship between high-fat diet-induced hepatic steatosis and the expression of GRP94, Caspase-3 and lncRNA-uc007gqg.1. Methods:Eighty 8-week-old New Zealand male white rabbits were randomly divided into control group (n=40) and experimental group (n=40). The blood biochemical indexes of 10 rabbits in each group were measured at the 1st, 4th, 6th and 8th week of the model, respectively. The pathological features of liver tissue were collected and the expression levels of lncRNA-uc007gqg.1 and GRP94,Caspase-3 protein in liver tissue were detected by real-time quantitative PCR and Westernblotting, respectively. Results:Compared with the control group, the levels of ALT,AST,TC,LDL,HDL and ALB,TB and CRP in the experimental group were significantly higher than those in the control group at the 4th weeks and 6th weeks, respectively. GGT,TG and the ratio of ALT to AST increased significantly at 6 weeks, and increased significantly at 8 weeks (P<0.01). The expression of lncRNA-uc007gqg.1 and GRP94,Caspase-3 protein increased gradually in liver tissue (all P<0.05). Conclusion:Uc007gqg.1 may be involved in the progression of NAFLD, suggests that uc007gqg. may be a new target for the prevention and treatment of NAFLD.
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