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叶黄素对H2O2作用下MIO-M1细胞活性及迁移的影响
作者:刘梦璇  马莉  何岁勤  陈文君  马翔 
单位:大连医科大学附属第一医院 眼科, 辽宁 大连 116011
关键词:叶黄素 MIO-M1细胞 氧化损伤 细胞迁移 
分类号:R774.5
出版年·卷·期(页码):2018·37·第六期(989-992)
摘要:

目的:探讨不同浓度叶黄素对过氧化氢(H2O2)作用下人眼Müller细胞系MIO-M1细胞活性及迁移的影响。方法:将MIO-M1细胞随机分为对照组、H2O2损伤组及叶黄素+H2O2共处理组,采用四甲基偶氮唑盐比色法(MTT法)及改良细胞划痕法检测不同浓度H2O2及加入叶黄素共处理后MIO-M1细胞活性及迁移率。结果:MTT法显示,与对照组相比,H2O2损伤组MIO-M1细胞活性显著抑制;与H2O2损伤组相比,加入叶黄素共处理后可增加MIO-M1细胞活性,差异均有统计学意义(P<0.05)。改良细胞划痕法表明,与对照组相比,H2O2损伤组MIO-M1细胞迁移率显著下降;与H2O2损伤组相比,加入叶黄素共处理后可提高MIO-M1细胞迁移率,差异均有统计学意义(P<0.05)。结论:叶黄素对H2O2作用下MIO-M1细胞活性及迁移具有保护作用。

Objective:To investigate the effect of different concentrations of lutein on viability and migration of human Müller cell line MIO-M1cells under hydrogen peroxide (H2O2) induced oxidative stress. Methods:MIO-M1 cells were randomly divided into control group, H2O2 group and co-treatment(lutein+H2O2) group. The viability and migration of MIO-M1 cells which were treated with different concentrations of H2O2 and co-treatment with lutein were analyzed by MTT assay and modified wound scratch assay.Results:MTT assay showed that the viability of MIO-M1 cells was significantly decreased in the H2O2 group compared with the control group. The viability of MIO-M1 cells in co-treatment group were significantly increased compared with the H2O2 group(all P<0.05).Modified wound scratch assay indicated that the migration rate of MIO-M1 cells was significantly decreased in the H2O2 group compared with control group. The migration rate of MIO-M1 cells in co-treatment group was significantly increased compared with H2O2 group(all P<0.05). Conclusion:Lutein can protect the viability and migration of MIO-M1cells from H2O2-induced oxidative stress.

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