枸杞多糖联合顺铂对肝癌小鼠抑瘤作用及其对免疫功能的影响-东南大学学报医学版

枸杞多糖联合顺铂对肝癌小鼠抑瘤作用及其对免疫功能的影响

单位：河南中医药大学, 河南郑州 450046

分类号：R735.7

出版年·卷·期（页码）：2018·37·第五期（896-900）

摘要：

目的：探讨枸杞多糖、顺铂及其联合使用对肝癌小鼠的抑瘤作用及对免疫功能的影响。方法：采用接种法制备小鼠H22实体瘤肝癌模型，40只成功建模的肝癌小鼠随机分为模型组、顺铂组、枸杞多糖组、联合组，每组10只。另取未造模小鼠10只作为对照组。给药14 d后记录各组小鼠的肿瘤重量，计算抑瘤率、脾脏指数和胸腺指数，检测IL-2和TNF-α水平及CD3⁺T、CD4⁺T、CD8⁺T细胞百分比。结果：与模型组比较，顺铂组小鼠脾脏指数和胸腺指数，IL-2、TNF-α水平，CD3⁺T、CD4⁺T细胞百分比，CD4⁺T/CD8⁺T值显著降低（P<0.05），枸杞多糖组、联合组小鼠的胸腺指数和脾脏指数，IL-2、TNF-α水平，CD3⁺T、CD4⁺T细胞百分比，CD4⁺T/CD8⁺T值显著升高（P<0.05）。与顺铂组比较，联合组小鼠的胸腺指数和脾脏指数，IL-2、TNF-α水平，CD3⁺T、CD4⁺T细胞百分比，CD4⁺T/CD8⁺T值显著升高（P<0.05）。结论：枸杞多糖联合顺铂可保护免疫器官，提高细胞因子水平，调节T淋巴细胞亚群平衡，减少顺铂对机体的免疫抑制作用，增强抗肿瘤疗效。

Objective:To investigate the effect of inhibitory and immune function by Lycium barbarum polysaccharide, cisplatin, and Lycium barbarum polysaccharide combined with cisplatin on mice with hepatic cancer. Methods:Forty hepatic cancer model in mice were established and randomly divided into model group, Lycium barbarum polysaccharide group, cisplatin group, combined group, ten mice in each groups. Another ten mice were chosen as blank control group. Spleen index and thymus index, IL-2, TNF-α,percentage of CD3⁺T, CD4⁺T, CD8⁺T cells in each groups were compared after administration. Results:Compared with model group, thymus index and spleen index, IL-2, TNF-α, CD3⁺T, CD4⁺T, CD4⁺T/CD8⁺T of the mice in cisplatin group were significantly decreased(P<0.05), meanwhile those were significant increased in Lycium barbarum polysaccharide group and combined group(P<0.05). Compared with cisplatin group, thymus index and spleen index, IL-2, TNF-α, CD3⁺T, CD4⁺T, CD4⁺T/CD8⁺T of the mice in combined group were significant increased (P<0.05). Conclusion:Lycium barbarum polysaccharide combined with cisplatin can enhance anti-tumor effect by protecting the immune organs, increasing the level of cytokine, regulating T lymphocyte subsets, decreasing the immunosuppression effect of cisplatin.

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