盐酸纳洛酮对急性呼吸窘迫综合征大鼠β-内啡肽及T淋巴细胞亚群的影响-东南大学学报医学版

盐酸纳洛酮对急性呼吸窘迫综合征大鼠β-内啡肽及T淋巴细胞亚群的影响

单位：郑州大学第一附属医院重症医学科, 河南郑州 450052

分类号：R563.8

出版年·卷·期（页码）：2018·37·第五期（824-828）

摘要：

目的：研究盐酸纳洛酮对急性呼吸窘迫综合征（ARDS）大鼠的免疫保护作用及其机制。方法：将30只健康SD大鼠随机分为对照组、模型组和纳洛酮组，每组10只。对照组：股静脉注射等量生理盐水；模型组：股静脉注射油酸（0.15 ml·kg^-1），2 h后注射生理盐水（1.0 ml·kg^-1）；纳洛酮组：股静脉注射油酸（0.15 ml·kg^-1），2 h后股静脉注射1.0 ml·kg^-1盐酸纳洛酮。计算左肺湿干比重，测定血浆中β-内啡肽、T淋巴细胞亚群含量，采用酶联免疫吸附法（ELISA）检验右肺匀浆TNF-α、IL-1β、IL-6、IL-8含量。结果：与对照组比较，ARDS模型组大鼠的左肺湿干比重、β-内啡肽、CD3⁺T细胞、CD4⁺T细胞、CD4⁺/CD8⁺T细胞、TNF-α、IL-1β、IL-6、IL-8水平均显著升高，CD8⁺T细胞显著降低（P<0.05）。与ARDS模型组比较，纳洛酮组大鼠的左肺湿干比重、β-内啡肽、CD3⁺T细胞、CD4⁺T细胞、CD4⁺/CD8⁺T细胞、TNF-α、IL-1β、IL-6、IL-8水平均显著降低，CD8⁺T细胞显著升高（P<0.05）。结论：盐酸纳洛酮通过抑制血浆β-内啡肽升高调节T淋巴细胞亚群平衡，降低机体炎症反应，减轻肺泡水肿，对ARDS起保护作用。

Objective:To investigate the immune protection and mechanism of naloxone hydrochloride on rats with acute respiratory distress syndrome (ARDS). Methods:Thirty healthy SD rats were randomly divided into control group, model group, and naloxone group,each group had ten rats. Control group:1.15 ml·kg^-1 normal saline was administrated by femoral vein injection. Model group:1.0 ml·kg^-1 normal saline was administrated by femoral vein injection after 0.15 ml·kg^-1 oleic acid was administrated. Naloxone group:1.0 ml·kg^-1 naloxone hydrochloride was administrated by femoral vein injection after 0.15 ml·kg^-1 oleic acid was administrated. The ratio of left lung wet/dry weight was calculated.β-endorphins and T-lymphocyte subsets in plasma was determined,and the content of TNF-α, IL-1β, IL-6, IL-8 were determined by enzyme linked immunosorbent assay(ELISA).Results:Compared with control group, Left lung wet/dry weight ratio, the level of β-endorphins, CD3⁺, CD4⁺, CD4⁺/CD8⁺, TNF-α, IL-1β, IL-6, IL-8 of rats in ARDS model group were significantly increased, CD8⁺ was significantly lower than control group(P<0.05).Compared with model group, Left lung wet/dry weight ratio, the level of β-endorphins,CD8+, TNF-α, IL-1β, IL-6, IL-8 of rats in naloxone group were significantly decreased, CD3⁺, CD4⁺ and CD4⁺/CD8⁺ were significantly increased (P<0.05).Conclusion:Naloxone hydrochloride can protect body from ARDS by inhibiting plasma β-Endorphins,adjecting T-lymphocyte subsets, reducing body inflammation and alveolar edema.

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