>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
小鼠下颌第一磨牙胚胎发育过程中的Notch信号表达
作者:居燕云  刘婷  苗雷英  汤旭娜 
单位:南京大学医学院 附属口腔医院/南京市口腔医院, 江苏 南京 210008
关键词:Notch1 Notch3 牙齿发育 细胞分化 小鼠 
分类号:Q959.837;Q954.48
出版年·卷·期(页码):2018·37·第四期(687-690)
摘要:

目的:探讨Notch1、Notch3在小鼠下颌第一磨牙胚胎发育过程中的组织学分布。方法:制作C57BL/6小鼠下颌第一磨牙不同发育阶段(自出生后14.5、18.5 d)的冰冻组织切片,进行Notch1、Notch3免疫组织化学染色,对其分布情况进行分析。结果:Notch1在小鼠下颌第一磨牙成釉器高表达,而成釉器周围聚集的间充质则未见明显表达;Notch3在小鼠下颌第一磨牙牙胚成釉器和成釉器周围聚集的间充质细胞中均有高表达。结论:Notch1和Notch3可能在小鼠下颌第一磨牙发育过程中的牙上皮,特别是内釉上皮的细胞分化、牙囊、牙乳头细胞分化及分化完成后的牙髓干细胞的稳定性方面有重要作用。

Objective:To investigate the expression of Notch1 and Notch3 in embryonic developing mouse mandibular first molar. Methods:The frozen sections of the first mandibular molars of C57BL/6 mice at different developmental stages (14.5-18.5 d after birth) were prepared for immunohistochemical staining of Notch1 and Notch3, and their distribution was analyzed. Results:Notch1 was highly expressed in the enamel formation of mouse first molar, but not in the mesenchymal cells around the enamel formation. Notch3 was highly expressed in mouse mandibular molar enamel and mesenchymal cells around the enamel. Conclusion:Notch1 and Notch3 may play an important role in the differentiation of dental epithelium, especially the inner enamel epithelium, the differentiation of dental follicle and dental papilla cells and the stability of dental pulp stem cells after the differentiation of mouse first molar teeth.

参考文献:

[1] LESOTH H,BROOK A H.Epithelial histogenesis during tooth development[J].Arch Oral Biol,2009,54(Suppl 1):S25-33.
[2] CAI X,GONG P,HUANG Y,et al.Notch signalling pathway in tooth development and adult dental cells[J].Cell Prolif,2011,44(6):495-507.
[3] BEI M.Molecular genetics of tooth development[J].Curr Opin Genet Dev,2009,19(5):504-510.
[4] YAN X,QIN H,QU C,et al.iPS cells reprogrammed from human mesenchymal-like stem/progenitor cells of dental tissue origin[J].Stem Cells Dev,2010,19(4):469-480.
[5] 刘铁军,刘健,陈砚凝,等.NF-κB、Notch-1及Jagged-1在口腔扁平苔藓中的表达及临床意义[J].中国现代医学杂志,2015,25(31):26-29.
[6] 王学玖,葛丽华,宿颖,等.Notch1及其配体Jagged1在小鼠颌下腺胚胎发育中的表达[J].北京口腔医学,2013,21(3):143-146.
[7] MA L,WANG S,TONG J,et al.Activation and dynamic expression of Notch signalling in dental pulp cells after injury in vitro and in vivo[J].Int Endod J,2016,49(12):1165-1174.
[8] HE F,YANG Z,TAN Y,et al.Effects of Notch ligand delta1 on the proliferation and differentiation of human dental pulp stem cells in vitro[J].Arch Oral Biol,2009,54(3):216-222.
[9] 毛丽莎,王学玖,葛丽华,等.Notch1在小鼠下颌第一磨牙牙胚发育中的表达[J].北京口腔医学,2014,22(5):251-254.
[10] ROTHOVA M,PETERKOVA R,TUCKER A S.Fate map of the dental mesenchyme:dynamic development of the dental papilla and follicle[J].Dev Biol,2012,366(2):244-254.
[11] URIBE-ETXEBARRIA V,LUZURIAGA J,AGLIANO A,et al.Notch/Wnt cross-signalling regulates stemness of dental pulp stem cells through expression of neural crest and core pluripotency factors[J].Eur Cell Mater,2017,34:249-270.
[12] MITSIADIS T A,CATON J,PAGELLA P,et al.Monitoring Notch signaling-associated activation of stem cell niches within injured dental pulp[J].Front Physiol,2017,8:372.
[13] MITSIADIS T A,REGAUDIAT L,GRIDLEY T,Role of the Notch signalling pathway in tooth morphogenesis[J].Arch Oral Biol,2005,50(2):137-140.
[14] MITSIADIS T A,HIRSINGER E,LENDAHL U,et al.Delta-notch signaling in odontogenesis:correlation with cytodifferentiation and evidence for feedback regulation[J].Dev Biol,1998,204(2):420-431.
[15] JAMAL M,CHOGLE S M,KARAM S M,et al.NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue[J].Genes Dis,2015,2(3):261-267.
[16] ZHANG C,CHANG J,SONOYAMA W,et al.Inhibition of human dental pulp stem cell differentiation by Notch signaling[J].J Dent Res,2008,87(3):250-255.
[17] 何飞,杨峥嵘,谭颖徽,等.Delta1基因传染人牙髓干细胞牙本质形成能力的研究[J].中国修复重建外科杂志,2007,21(10):1133-1136.
[18] WANG X,HE F,TAN Y H,et al.Inhibition of Delta1 promotes differentiation of odontoblasts and inhibits proliferation of human dental pulp stem cell in vitro[J].Arch Oral Biol,2011,56(9):837-845.
[19] MITSIADIS T A,GRAF D,LUDER H,et al.BMPs and FGFs target Notch signalling via jagged 2 to regulate tooth morphogenesis and cytodifferentiation[J].Development,2010,137(18):3025-3035.
[20] FAUX C H,TURNLEY A M,EPA R,et al.Interactions between fibroblast growth factors and Notch regulate neuronal differentiation[J].J Neurosci,2001,21(15):5587-5596.
[21] NICOLAS M,WOLFER A,RAJ K,et al.Notch1 functions as a tumor suppressor in mouse skin[J].Nat Genet,2003,33(3):416-421.
[22] INGRAM W J,MCCUE K I,TRAN T H,et al.Sonic Hedgehog regulates Hes1 through a novel mechanism that is independent of canonical Notch pathway signaling[J].Oncogene,2008,27(10):1489-1500.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 405495 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-83272481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364