miR-182在子宫内膜癌中表达及其对子宫内膜癌细胞增殖和凋亡的影响-东南大学学报医学版

miR-182在子宫内膜癌中表达及其对子宫内膜癌细胞增殖和凋亡的影响

单位：济宁医学院附属医院妇产科, 山东济宁 272100

分类号：R737.33

出版年·卷·期（页码）：2018·37·第四期（653-659）

摘要：

目的：检测miR-182在子宫内膜癌组织中的表达，探讨其对子宫内膜癌细胞增殖、凋亡的影响及其机制。方法：用real-time PCR检测子宫内膜正常组织和癌组织中miR-182的表达；体外使用miR-182抑制剂和（或）si-Foxo1转染子宫内膜癌细胞RL95-2，用MTT和Annexin V-FITC/PI双染法及Western blot分别检测细胞增殖和凋亡水平以及Cyclin D1、caspase3和肿瘤抑制基因Foxo1的表达。结果：miR-182在子宫内膜癌组织中的表达较正常组织显著升高（P<0.05）；下调miR-182可抑制RL95-2细胞的增殖活性，增加细胞凋亡百分比，同时可促使Cyclin D1、active caspase3和Foxo1的表达增加。抑制Foxo1可部分逆转miR-182抑制剂的作用。结论：miR-182在子宫内膜癌中表达异常增高；miR-182异常表达可通过抑制Foxo1表达促使RL95-2细胞的增殖，并抑制其凋亡。

Objective:To detect the expression of miR-182 in endometrial carcinoma tissues and investigate the effects and mechanisms of miR-182 on the proliferation and apoptosis of endometrial carcinoma cells. Methods:The expression of miR-182 was detected in endometrial carcinoma tissues and normal endometrial tissues by real-time PCR. Endometrial cancer cells RL95-2 were transfected with miR-182 inhibitor and/or si-Foxo1 in vitro. Then the proliferative activity and apoptotic percentage of cells were measured by MTT assay and Annexin V-FITC/PI double staining assay; the expression of Cyclin D1 and Foxo1 were detected by Western blot. Results:Compared with normal endometrial tissues, the expression of miR-182 in endometrial carcinoma was significantly increased (P<0.05). Downregulation of miR-182 could significantly inhibit cell proliferation, increase the percentage of apoptosis and promote the expression of Foxo1, Cyclin D1 and active caspase3. But the inhibition of Foxo1 expression could partially reverse these effects of miR-182 inhibitor. Conclusion:miR-182 was abnormally high expressed in endometrial carcinoma, and its abnormal expression could promote cell proliferation and inhibit apoptosis of RL95-2 cells by inhibiting Foxo1 expression.

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