粉防己碱对胃癌耐药细胞ZNF139、MDR1及GST-π mRNA的影响-东南大学学报医学版

粉防己碱对胃癌耐药细胞ZNF139、MDR1及GST-π mRNA的影响

作者：张旭

单位：内江市第一人民医院消化内科, 四川内江 641000

分类号：R735.2

出版年·卷·期（页码）：2018·37·第三期（467-470）

摘要：

目的：探讨粉防己碱（TET）对胃癌耐药细胞锌指蛋白139（ZNF139）、多耐药基因1（MDR1）及谷胱甘肽S转移酶-π（GST-π） mRNA的影响。方法：选取胃癌细胞SGC7901以及胃癌耐阿霉素细胞SGC7901/ADR，采用逆转录-聚合酶链反应和蛋白质印迹法检测两种细胞ZNF139、MDR1、GST-π mRNA及蛋白表达，同时检测2.0 μg·ml^-1 TET处理前后SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA及蛋白表达。结果：SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA相对表达量明显高于SGC7901细胞（P<0.05）；SGC7901/ADR细胞ZNF139、MDR1、GST-π蛋白相对表达量明显高于SGC7901细胞（P<0.05）；TET处理后SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA及蛋白相对表达量均较处理前明显降低（P<0.05）。结论：TET能下调胃癌耐药细胞ZNF139、MDR1、GST-π mRNA和蛋白表达水平，从而在逆转胃癌多药耐药特性方面有重要作用。

Objective: To investigate the effects of tetrandrine(TET) on zinc finger protein 139 (ZNF139), multidrug resistance gene 1(MDR1) and glutathione S transferase-π(GST-π) mRNA of multidrug resistant gastric cancer cells.Methods: Gastric cancer cells SGC7901 and SGC7901/ADR were selected, and ZNF139, MDR1 and GST-π mRNA and protein expression in the two kinds of cells were detected by RT-PCR and Western blot. ZNF139, MDR1 and GST-π mRNA and protein expression of SGC7901/ADR cells were detected before and after 2.0 μg·ml^-1 TET treatment.Results: ZNF139, MDR1 and GST-π mRNA relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells (P<0.05); ZNF139, MDR1 and GST-π protein relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells(P<0.05); After TET treatment ZNF139, MDR1 and GST-π mRNA and protein relative expression of SGC7901/ADR cells were significantly decreased compared with those before treatment(P<0.05).Conclusion: TET can down-regulate ZNF139, MDR1 and GST-π mRNA and protein expression in drug resistant gastric cancer cells, which plays an important role in reversing multidrug resistance characteristics of gastric cancer.

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