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粉防己碱对胃癌耐药细胞ZNF139、MDR1及GST-π mRNA的影响
作者:张旭 
单位:内江市第一人民医院 消化内科, 四川 内江 641000
关键词:胃癌 粉防己碱 锌指蛋白139 多耐药基因1 谷胱甘肽S转移酶-π 耐药 
分类号:R735.2
出版年·卷·期(页码):2018·37·第三期(467-470)
摘要:

目的:探讨粉防己碱(TET)对胃癌耐药细胞锌指蛋白139(ZNF139)、多耐药基因1(MDR1)及谷胱甘肽S转移酶-π(GST-π) mRNA的影响。方法:选取胃癌细胞SGC7901以及胃癌耐阿霉素细胞SGC7901/ADR,采用逆转录-聚合酶链反应和蛋白质印迹法检测两种细胞ZNF139、MDR1、GST-π mRNA及蛋白表达,同时检测2.0 μg·ml-1 TET处理前后SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA及蛋白表达。结果:SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA相对表达量明显高于SGC7901细胞(P<0.05);SGC7901/ADR细胞ZNF139、MDR1、GST-π蛋白相对表达量明显高于SGC7901细胞(P<0.05);TET处理后SGC7901/ADR细胞ZNF139、MDR1、GST-π mRNA及蛋白相对表达量均较处理前明显降低(P<0.05)。结论:TET能下调胃癌耐药细胞ZNF139、MDR1、GST-π mRNA和蛋白表达水平,从而在逆转胃癌多药耐药特性方面有重要作用。

Objective: To investigate the effects of tetrandrine(TET) on zinc finger protein 139 (ZNF139), multidrug resistance gene 1(MDR1) and glutathione S transferase-π(GST-π) mRNA of multidrug resistant gastric cancer cells.Methods: Gastric cancer cells SGC7901 and SGC7901/ADR were selected, and ZNF139, MDR1 and GST-π mRNA and protein expression in the two kinds of cells were detected by RT-PCR and Western blot. ZNF139, MDR1 and GST-π mRNA and protein expression of SGC7901/ADR cells were detected before and after 2.0 μg·ml-1 TET treatment.Results: ZNF139, MDR1 and GST-π mRNA relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells (P<0.05); ZNF139, MDR1 and GST-π protein relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells(P<0.05); After TET treatment ZNF139, MDR1 and GST-π mRNA and protein relative expression of SGC7901/ADR cells were significantly decreased compared with those before treatment(P<0.05).Conclusion: TET can down-regulate ZNF139, MDR1 and GST-π mRNA and protein expression in drug resistant gastric cancer cells, which plays an important role in reversing multidrug resistance characteristics of gastric cancer.

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