糖尿病大鼠髌腱来源肌腱干细胞体外成脂分化潜能的实验研究-东南大学学报医学版

糖尿病大鼠髌腱来源肌腱干细胞体外成脂分化潜能的实验研究

作者：徐宏亮¹ 2 石柳³ 4 5 李荥娟⁶ 徐林¹ 2 王宸¹ 2 3 4 陈辉¹ 2 3 4 芮云峰¹ 2 3 4

单位：1. 无锡市锡山人民医院骨科, 江苏无锡 214000;
2. 东南大学附属中大医院无锡分院骨科, 江苏无锡 214000;
3. 东南大学附属中大医院骨科, 江苏南京 210009;
4. 东南大学创伤骨科研究所, 江苏南京 210009;
5. 香港中文大学矫形外科及创伤学系, 香港;
6.

关键词：糖尿病髌腱肌腱干细胞成脂分化大鼠

分类号：R-33;R587.1

出版年·卷·期（页码）：2018·37·第三期（390-395）

摘要：

目的：观察糖尿病大鼠髌腱来源的肌腱干细胞（TDSCs）体外成脂分化的潜能，探讨糖尿病对TDSCs成脂分化潜能的影响。方法：采用链脲佐菌素建立糖尿病大鼠模型，提取大鼠髌腱TDSCs；观察细胞形态，并在体外诱导其成脂分化，通过油红O染色和检测成脂分化相关基因（C/EBP和PPARγ2）的mRNA表达，比较糖尿病组与对照组TDSCs成脂分化潜能的差异。结果：造模后糖尿病组血糖水平显著高于对照组，并持续高于250 mg·dl^-1。对照组TDSCs （hTDSCs）形态在镜下表现为均一的细长纺锤型，而糖尿病组TDSCs （dTDSCs）表现为多突的纺锤型。成脂分化诱导9 d后，经油红O染色，对照组细胞内可见大量的脂滴形成，而糖尿病组细胞内仅有少量的脂滴形成，dTDSCs油红O染色阳性率显著低于对照组。dTDSCs C/EBP、PPARγ2的mRNA水平也显著低于hTDSCs。结论：糖尿病TDSCs体外成脂分化能力较hTDSCs显著降低，此结果为进一步揭示糖尿病肌腱病变过程中，持续体内高糖环境对TDSCs成脂分化功能影响的潜在分子机制提供了细胞生物学依据。

Objective: To investigate the adipogenic differentiation potential of tendon-derived stem cells(TDSCs) in diabetes mellitus rats.Methods: The diabetic rats were induced by streptozotocin and the TDSCs were isolated at week 2 post induction. The morphology of both healthy TDSCs(hTDSCs) and diabetic TDSCs(dTDSCs) was photographed under light microscopy. Oil Red O staining and C/EBP and PPARγ2 expression level were measured by qRT-PCR at day 9 post adipogenic induction.Results: Blood glucose levels of diabetic rats were sustained higher than 250 mg·dl^-1 after STZ injection. Both kinds of the TDSCs presented the slender spindle shaped morphology while there was a little change in dTDSCs. Significantly decreased oil red O-positive droplets in dTDSCs than hTDSCs were discovered. qRT-PCR results also indicated that the mRNA expressions of C/EBP and PPARγ2 in the TDSCs from the diabetic tendons were higher than those in hTDSCs.Conclusion: TDSCs from STZ induced diabetic rats show more significantly decreased adipogenic differentiation potential than those from healthy controls, which might contribute to better understanding of the pathogenesis of diabetic tendinopathy.

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