瓜蒌皮提取物对同型半胱氨酸诱导动脉粥样硬化的保护作用-东南大学学报医学版

瓜蒌皮提取物对同型半胱氨酸诱导动脉粥样硬化的保护作用

单位：1. 莱芜市人民医院血管外科, 山东莱芜 271100;
2. 郑州市第八人民医院药械科, 河南郑州 450045;
3. 河南省中医院中心实验室, 河南郑州 450045

分类号：R543.5;R-33

出版年·卷·期（页码）：2018·37·第二期（239-243）

摘要：

目的：探讨在大鼠中瓜蒌皮提取物如何影响同型半胱氨酸诱导的动脉粥样硬化。方法：将60只Wistar大鼠随机分为3组：对照组20只、同型半胱氨酸模型组20只、瓜蒌皮提取物实验组20只，采取1%蛋氨酸喂养诱导动脉粥样硬化模型。检测血清中TC、TG、LDL、Hcy浓度，主动脉组织病理切片观察斑块情况，MTT试剂检测同型半胱氨酸、瓜蒌皮提取物处理后对血管平滑肌细胞增殖影响，RT-PCR检测同型半胱氨酸、瓜蒌皮提取物处理后血管平滑肌细胞内c-myc、c-fos基因的表达情况。结果：同型半胱氨酸模型组血清中TC、TG、LDL、Hcy浓度显著高于实验组和对照组（P<0.05），实验组和对照组差异无统计学意义（P>0.05）。主动脉组织切片显示实验组动脉粥样硬化斑块得到显著改善。MTT细胞增殖检测发现，高同型半胱氨酸处理血管平滑肌细胞后，细胞增殖速度显著高于对照组和实验组（P<0.05），而实验组和对照组间差异无统计学意义（P>0.05）。高同型半胱氨酸处理血管平滑细胞后，细胞内c-myc、c-fos基因表达显著高于对照组和实验组（P<0.05），而实验组和对照组间差异无统计学意义（P>0.05）。结论：瓜蒌皮提取物可以抑制同型半胱氨酸诱导的动脉粥样硬化。

Objective:To investigated the effect of extractive of pericarpium trichosanthis (EPT) in atherosclerosis induced by homocysteine in rats. Methods:60 Wistar rats were randomly divided into three groups:control group, homocysteine model group, EPT experimental group, and 20 rats in each group. And 1% methionine diet was fed to rats to induce atherosclerosis model. The serum TC, TG, LDL and Hcy concentration were detected by instrument. The change of aortic plaque was observed from aorta tissue pathological slices. The effect on the proliferation of vascular smooth muscle cells after treatment of homocysteine and EPT was detected by MTT. The expression of c-myc and c-fos gene in vascular smooth muscle cells after treatment of homocysteine and EPT was detected by RT-PCR. Results:The serum concentrations of TC, TG, LDL and Hcy in the model group were significantly higher than those in the experimental group and the control group (P<0.05), and there was no significant difference between the experimental group and the control group (P>0.05). The result of aortic tissue sections showed that the atherosclerotic plaque in the experimental group improved significantly. MTT cell proliferation detection showed that after high homocysteine treatment of vascular smooth muscle cells, the cell proliferation rate was significantly higher than that of the control group and the experimental group (P<0.05), while the experimental group and the control group had no statistical difference (P>0.05). After high homocysteine treatment of vascular smooth cells, the expression of c-myc and c-fos in the cells was significantly higher than that in the control group and the experimental group (P<0.05), but there was no significant difference between the experimental group and the control group (P>0.05). Conclusion:EPT can inhibit atherosclerosis induced by homocysteine.

参考文献：

[1] 杨发满,黄新,敬泽慧.瓜蒌皮注射液治疗老年急性缺血性脑病患者应用分析[J].时珍国医国药,2015(6):1415-1417.
[2] 郭方圆.丹蒌片对兔颈动脉粥样硬化斑块的影响[D].上海:第二军医大学,2012.
[3] 赵志英.血脂与脑梗死患者颈动脉粥样硬化的关系[J].检验医学,2015,28(47):388-389.
[4] 王冬梅,代世元,芦丽莉,等.瓜蒌皮提取物对大鼠动脉粥样硬化保护作用的实验研究[J].北华大学学报:自然版,2008,9(2):128-131.
[5] 刘姿麟,林慕之,况春燕,等.大鼠主动脉血管平滑肌细胞原代培养与鉴定[J].贵州医科大学学报,2017,42(2):125-129.
[6] 黄鑫涛,李之恒,李中原,等.冠状动脉粥样硬化性心脏病与血浆同型半胱氨酸发病的相关性研究[J].中国医学工程,2015(1):117.
[7] 李知莲,吴慧芳.血清同型半胱氨酸水平与急性脑梗死患者颈动脉粥样硬化的相关性[J]. 实用临床医药杂志,2015,19(7):141-142.
[8] 刘迎春,吴德云,高宗恩,等.高血压患者颈动脉粥样硬化与同型半胱氨酸的相关性研究[J]. 中国循证医学杂志,2015(5):503-507.
[9] 施洪,郭润达,刘辉强,等.颈动脉粥样硬化与同型半胱氨酸、血脂、血糖、尿酸相关性研究[J].中国实用神经疾病杂志,2015(1):38-40.
[10] 程清.脂蛋白相关磷脂酶A2和同型半胱氨酸水平与动脉粥样硬化性心脑血管疾病关系的研究[J].检验医学,2015,30(1):40-43.
[11] 梁顺今,崔英花,李红梅,等.高同型半胱氨酸血症与颈动脉粥样硬化关系分析[J].中国实用神经疾病杂志,2012,15(21):51-52.
[12] 王恰如,王晓霞.颈动脉粥样硬化与高同型半胱氨酸相关性的研究进展[J].实用心脑肺血管病杂志,2012,20(3):389-391.
[13] 常娜,鲁广秀,张建磊.同型半胱氨酸与动脉粥样硬化型急性脑梗死患者颈动脉粥样硬化的相关性[J].实用医学杂志,2013,29(1):69-70.
[14] 邵振兴,孙金凤.血清同型半胱氨酸水平与颈动脉粥样硬化的关系探讨[J].海南医学,2012,23(4):102-103.
[15] 曾双龙,徐俊波,胡咏梅.同型半胱氨酸与冠状动脉粥样硬化性心脏病的相关性研究进展[J].心血管病学进展,2012,33(5):650-653.
[16] 杨静,刘德山,高伟,等.大蒜素对兔血管平滑肌细胞增殖及c-myc基因表达的影响[J].中国老年学,2011,31(4):622-624.
[17] 邹治铭,郭华雄.C-myc在肿瘤中作用的研究进展[J].现代医学,2016,44(7):1024-1026.
[18] 席锐,邹琛,陈桢玥,等.同型半胱氨酸促进血管平滑肌细胞增殖的机制及辛伐他汀的干预作用[J].国际心血管病杂志,2015,42(1):44-47.

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