Objective:To investigate the protective effect and mechanism of lycopene (Lyc) on damage of mouse podocytes (MPC5) induced by high glucose. Methods:MPC5 were cultured in vitro and treated with high glucose and different doses of Lyc (3.125, 6.25, 12.5μmol·L-1). Then cell viabilties and intracellular ROS content were detected by MTT and flow cytometry, respectively; and the expression levels of marker proteins for cell damage, autophagy related proteins and some key proteins involved in phosphatidylinositol 3 kinase/protein kinase B (PI3K/AKT) signaling pathway were detected by Western blot. Results:Compared with that in normal glucose group, cell viability of MPC5 cells in high glucose group decreased while the ROS content increased in high glucose group (P<0.05). Lyc increased cell viability of MPC5 cells in high glucose state, and this effect was dose-dependent. Lyc treatment could decrease intracellular ROS contents, induce autophagy increasing, improve cell damage and activate PI3K/AKT signaling pathway. Compared with those in high glucose group, all of those differences were significant (P<0.05). Compared with high glucose and high dose of Lyc group, the expression levels of proteins involved in PI3K/AKT pathway were inhibited, cell autophagy decreased, cell injury increased (P<0.05) after the treatment with LY294002. Conclusion:Lyc can relieve the damage of MPC5 induced by high glucose through reducing the ROS content and enhancing the autophagy of MPC5 cells, which the mechanism may be related to PI3K/AKT signaling pathway.
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