Objective:To identify the role of paeoniflorin(Pae) on attenuating Ang-Ⅱ induced cardiomyocyte hypertrophy. Methods:A model of cardiac hypertrophy induced by Ang-Ⅱ in vitro was developed. Cardiomyocytes were separated and divided into four groups:control group, Ang-Ⅱ group, Pae group and Ang-Ⅱ plus Pae group. Cells were transfected with miR-21 mimic, Smad7 siRNA or their negative control group. Myocardial cell image analysis system was used to measure the relative surface area of cardiomyocytes; Real-time PCR was used to assay the mRNA expression of miR-21, ANP and BNP. A dual luciferase reporter assay was used to detect the relationship between Smad7 activity and miR-21 expression.Western blotting was used to evaluate the protein level of Smad7, ANP and BNP. Results:Ang Ⅱ pretreatment significantly increased the myocardial cell surface area, as well as up-regulated the miR-21, ANP and BNP expression, while paeoniflorin can partly reverse these effects brought by Ang Ⅱ (all P<0.05). Results of dual luciferase reporter assay showed that miR-21 directly targeted the 3'-UTR of Smad7, resulting in the post-transcriptional translation inhibition of Smad7 (P<0.05). Pae can partly reduce the inhibition effect of miR-21 on Smad7 (P<0.05), Smad7 knockdown remarkably attenuated the inhibition effect on cell relative surface area, ANP and BNP expression induced by Pae (P<0.05). Conclusion:These results indicate that Pae may resistance to Ang-Ⅱ induced cardiac hypertrophy via miR-21/Smad7 pathway.
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