Objective:To explore the effect of FoxO1 inhibition in the expressions of IL-1β in the diabetic rats retina induced by STZ and its mechanism. Methods:The human retinal capillary endothelial cells (HRCECs) were treated by different concentration of glucose. The expressions of FoxO1, IL-1β were measured by RT-PCR and Western blot. The HRCECs were divided into Mock group, NC-FoxO1 group and si-FoxO1 group, the expressions of FoxO1, IL-1β mRNA were measured by RT-PCR, the expressions of FoxO1, IL-1β, p-P38, p-JNK, p-ERK protein were measured by Western blot. 60 rats were randamly divided into Control group, DM group, LV-si-FoxO1 group and LV-NC group, the diabetic rats models were established in DM group, LV-si-FoxO1 group and LV-NC group. After models were estabilished sucessfully, the LV-si-FoxO1 or LV-NC lentiviral vector was injected in the vitreous cavity of diabetic rats, the retinal tissue was completely separated at the end of 12 weeks, the expressions of FoxO1, IL-1β in retinal tissue were measured by immunohistochemical staining, RT-PCR and Western blot. Results:Compared to the 5 mmol·L-1 glucose group, the expressions of FoxO1, IL-1β mRNA and protein in 15 mmol·L-1 glucose group had no significant differences (P>0.05), the expressions of FoxO1, IL-1β mRNA and protein in 25 mmol·L-1 glucose group were significantly higher than those in 5 mmol·L-1 glucose group, and FoxO1, IL-1β mRNA and protein in 35 mmol·L-1 glucose group increased more obviously, the difference between groups was statistically significant (P<0.05). The expressions of IL-1β mRNA and protein in si-FoxO1 group were significantly lower than those in NC-FoxO1 group and Mock group (P<0.05), there were no significant differences of IL-1β mRNA and protein between NC-FoxO1 group and Mock group (P>0.05). The expressions of FoxO1, IL-1β mRNA and protein in DM group and LV-NC group were significantly higher than those in Control group and LV-si-FoxO1 group (P<0.05), there were no significant differences of FoxO1, IL-1β mRNA and protein between LV-NC group and Control group(P>0.05), there were also no significant differences between DM group and LV-NC group (P>0.05). The expressions of p-P38, p-JNK, p-ERK protein in si-FoxO1 group were significantly lower than those in NC-FoxO1 group and Mock group (P<0.05), and the expressions of p-P38, p-JNK, p-ERK protein between NC-FoxO1 group and Mock group had no significant differences (P>0.05). Conclusion:High glucose can promote the expressions of FoxO1, IL-1β in HRCECs, inhibition the expression of FoxO1 may be done by down-reuglation of IL-1β expression through decreasing the MAPK phosphorylation.
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