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hsa_circ_RNA0023397和miR-106b在活动性克罗恩病肠黏膜组织中的表达及意义
作者:沈朵1  陈洪2  李红霞1  徐孝新1  尹莹2 
单位:1. 东南大学 医学院, 江苏 南京 210009;
2. 东南大学附属中大医院 消化科, 江苏 南京 210009
关键词:克罗恩病 hsa_circ_0023397 miR-106b ATG16L1 自噬 
分类号:R574
出版年·卷·期(页码):2018·37·第一期(22-27)
摘要:

目的:检测hsa_circ_0023397和miR-106b在克罗恩病(Crohn's disease,CD)中的表达及其与自噬的关系,为CD的分子诊断和靶向治疗提供新思路。方法:在环状RNA芯片检测的基础上,收集活动期CD患者及健康体检者各40例,采用实时定量PCR检测肠黏膜组织中hsa_circ_0023397和miR-106b的表达情况,并用Western Blot检测自噬相关蛋白ATG16L1及LC3-Ⅱ/LC3-I的表达情况。结果:与健康体检组相比,活动期CD患者肠黏膜组织中hsa_circ_0023397明显下降(P < 0.01),而miR-106b显著升高(P < 0.01)。Western Blot检测发现,活动期CD肠黏膜组织中ATG16L1及LC3-Ⅱ/LC3-I比值均下降(P<0.05)。结论:活动期CD患者肠黏膜组织中可能存在着由于hsa_circ_0023397下调引起miR-106b过度表达,继而导致ATG16L1表达下调并降低CD患者自噬功能的新机制,有待进一步研究。

Objective: To investigate the expression of hsa_circ_0023397 and miR-106b in Crohn's disease (CD) and its relationship with autophagy and to provide new ideas for the molecular diagnosis and targeted therapy of CD. Methods: Based on the circRNA chip detection, hsa_circ_0023397 and miR-106b expresssion levels were analysed in 40 active CD patients and 40 health controls by the real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR). The expression of autophagy related protein ATG16L1 and LC3-Ⅱ/LC3-I were assessed by Western Blot. Results: Compared with the healthy controls, the expression of hsa_circ_0023397 decreased significantly in active CD patients (P<0.01), whereas miR-106b expression level increased (P<0.01). Western Blot analysis revealed that the expression of ATG16L1 and LC3-Ⅱ/LC3-I decreased in active CD (P<0.05). Conclusion: There may be a new mechanism that remains to be further studied for the overexpression of miR-106b due to the down-regulation of hsa_circ_0023397, leading to the down-regulation of ATG16L1 expression and reduction of the autophagy in the intestinal mucosal tissues of patients with active CD.

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