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姜黄素通过内质网应激信号通路诱导肝癌SMMC-7721细胞凋亡的作用研究
作者:徐新明1 2  陈健1  陆荣柱2  刘瑞文1  冯浩1  张明华1 
单位:1. 江苏大学附属昆山医院 肝胆外科, 江苏 苏州 215300;
2. 江苏大学 医学院, 江苏 镇江 212013
关键词:肝癌 姜黄素 内质网应激 凋亡 
分类号:R735.7
出版年·卷·期(页码):2018·37·第一期(12-17)
摘要:

目的:检测姜黄素对肝癌SMMC-7721细胞增殖及凋亡的影响,并进一步探讨内质网应激反应在其中的作用。方法:应用2.5 μM、5 μM、10 μM、20 μM、50 μM的姜黄素处理肝癌SMMC-7721细胞,采用四甲基偶氮唑蓝(MTT)法检测细胞活性;Western Blot检测内质网应激相关蛋白GRP78、caspase-12、CHOP的表达;流式细胞术检测姜黄素对肝癌细胞凋亡的影响。结果:与对照组比较,2.5 μM、5 μM、10 μM、20 μM、50 μM的姜黄素对肝癌SMMC-7721细胞增殖均有抑制作用,并随作用时间的增加,增殖抑制作用更加显著(均P<0.05);姜黄素能够诱导肝癌SMMC-7721细胞GRP78(P<0.05)、caspase-12(P<0.05)、CHOP(P<0.01)的表达,其表达量具有剂量依赖性;姜黄素能够诱导肝癌SMMC-7721细胞凋亡,与对照组比较(P<0.05),呈现出剂量依赖关系。结论:姜黄素通过内质网应激信号通路诱导肝癌细胞凋亡,抑制其增殖。

Objective: To investigate the effect of curcumin on ERS-related apoptosis and cell proliferation in smmc-7721 cells. Methods: SMMC-7721 cells were incubated with the blank vehicle, 2.5, 5, 10, 20 μM of curcumin. After intervention, cell proliferation were detected by MTT, protein expressions of GRP78, caspase-12, CHOP were assessed by western blotting. Cell apoptotic rate were detected by flow cytometry. Results: Compared with the control group, 2.5, 5, 10, 20 μM cucurmin inhibited the proliferation of hepatocellular carcinoma SMMC-7721 cells, and with the increase of the time, the inhibition of proliferation is more significant(All P<0.05). Curcumin could induce the expression of GRP78(P<0.05), caspase-12(P<0.05) and CHOP(P<0.01) in SMMC-7721 cells, and the expression of these proteins structures were dose dependent. Curcumin induced apoptosis of hepatocellular carcinoma SMMC-7721 cells, compared with the control group(P<0.05), showing a dose-dependent relationship. Conclusion: Curcumin may suppresse cell proliferation and induce apoptosis partly through activation of ERS signaling pathway in hepatocellular carcinoma cells.

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