Objective: To investigate the effect of curcumin on ERS-related apoptosis and cell proliferation in smmc-7721 cells. Methods: SMMC-7721 cells were incubated with the blank vehicle, 2.5, 5, 10, 20 μM of curcumin. After intervention, cell proliferation were detected by MTT, protein expressions of GRP78, caspase-12, CHOP were assessed by western blotting. Cell apoptotic rate were detected by flow cytometry. Results: Compared with the control group, 2.5, 5, 10, 20 μM cucurmin inhibited the proliferation of hepatocellular carcinoma SMMC-7721 cells, and with the increase of the time, the inhibition of proliferation is more significant(All P<0.05). Curcumin could induce the expression of GRP78(P<0.05), caspase-12(P<0.05) and CHOP(P<0.01) in SMMC-7721 cells, and the expression of these proteins structures were dose dependent. Curcumin induced apoptosis of hepatocellular carcinoma SMMC-7721 cells, compared with the control group(P<0.05), showing a dose-dependent relationship. Conclusion: Curcumin may suppresse cell proliferation and induce apoptosis partly through activation of ERS signaling pathway in hepatocellular carcinoma cells. |
[1] LAU W Y,SANGRO B,CHEN P J,et al.Treatment for hepatocellular carcinoma with portal vein tumor thrombosis:the emerging role for radioembolization using yttrium-90[J].Oncology,2013,84(5):311-318.
[2] HERNANDEZ-GEA V,TOFFANIN S,FRIEDMAN S L,et al.Role of the microenvironment in the pathogenesis and treatment of hepatocellular carcinoma[J].Gastroenterology,2013,144(3):512-527.
[3] MENON L G,KUTTAN R,KUTTAN G.Inhibition of lung metastasis in mice induced by B16F10 melanoma cells by polyphenolic compounds[J].Cancer Lett,1995,95(1-2):221-225.
[4] 张君,童钟,李迎霞,等.姜黄素对肿瘤细胞增殖的影响[J].安徽医药,2014(08):1545-1548.
[5] 邱伟,刘阳,曹卫,等.姜黄素抑制肝癌细胞系HepG2的增殖、侵袭及其机制[J].现代肿瘤医学,2015(22):3221-3225.
[6] 范双娜,卢洁.姜黄素与顺铂联合应用对胃癌SGC-7901的体外抑制作用[J].实用药物与临床,2016(02):135-139.
[7] LI L,AGGARWAL B B,SHISHODIA S,et al.Nuclear factor-kappaB and IkappaB kinase are constitutively active in human pancreatic cells,and their down-regulation by curcumin(diferuloylmethane) is associated with the suppression of proliferation and the induction of apoptosis[J].Cancer,2004,101(10):2351-2362.
[8] FERLAY J,SHIN H R,BRAY F,et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008[J].Int J Cancer,2010,127(12):2893-2917.
[9] KUNNUMAKKARA A B,ANAND P,AGGARWAL B B.Curcumin inhibits proliferation,invasion,angiogenesis and metastasis of different cancers through interaction with multiple cell signaling proteins[J].Cancer Lett,2008,269(2):199-225.
[10] 张君,童钟,李迎霞,等.姜黄素对肿瘤细胞增殖的影响[J].安徽医药,2014(08):1545-1548.
[11] 李一诗,傅仲学.姜黄素逆转肿瘤多药耐药的研究进展[J].重庆医学,2010(16):2225-2227.
[12] KIKUCHI H,KURIBAYASHI F,MIMURO H,et al.Lack of GCN5 remarkably enhances the resistance against prolonged endoplasmic reticulum stress-induced apoptosis through up-regulation of Bcl-2 gene expression[J].Biochem Biophys Res Commun,2015,463(4):870-875.
[13] LIU D,ZHANG M,YIN H.Signaling pathways involved in endoplasmic reticulum stress-induced neuronal apoptosis[J].Int J Neurosci,2013,123(3):155-162.
[14] TABAS I,RON D.Integrating the mechanisms of apoptosis induced by endoplasmic reticulum stress[J].Nat Cell Biol,2011,13(3):184-190.
[15] ARBABIAN A,BROULAND J P,GELEBART P,et al.Endoplasmic reticulum calcium pumps and cancer[J].Biofactors,2011,37(3):139-149.
[16] JONES P G,NAWOSCHIK S P,SREEKUMAR K,et al.Tissue distribution and functional analyses of the constitutively active orphan G protein coupled receptors,GPR26 and GPR78[J].Biochim Biophys Acta,2007,1770(6):890-901.
[17] ROBERSON E C,TULLY J E,GUALA A S,et al.Influenza induces endoplasmic reticulum stress,caspase-12-dependent apoptosis,and c-Jun N-terminal kinase-mediated transforming growth factor-beta release in lung epithelial cells[J].Am J Respir Cell Mol Biol,2012,46(5):573-581.
[18] 袁小青,马向华,沈捷.核转录因子CHOP研究进展[J].医学研究杂志,2008(08):15-18. |