Objective: To investigate the effect of curcumin on ERS-related apoptosis and cell proliferation in smmc-7721 cells. Methods: SMMC-7721 cells were incubated with the blank vehicle, 2.5, 5, 10, 20 μM of curcumin. After intervention, cell proliferation were detected by MTT, protein expressions of GRP78, caspase-12, CHOP were assessed by western blotting. Cell apoptotic rate were detected by flow cytometry. Results: Compared with the control group, 2.5, 5, 10, 20 μM cucurmin inhibited the proliferation of hepatocellular carcinoma SMMC-7721 cells, and with the increase of the time, the inhibition of proliferation is more significant(All P<0.05). Curcumin could induce the expression of GRP78(P<0.05), caspase-12(P<0.05) and CHOP(P<0.01) in SMMC-7721 cells, and the expression of these proteins structures were dose dependent. Curcumin induced apoptosis of hepatocellular carcinoma SMMC-7721 cells, compared with the control group(P<0.05), showing a dose-dependent relationship. Conclusion: Curcumin may suppresse cell proliferation and induce apoptosis partly through activation of ERS signaling pathway in hepatocellular carcinoma cells.
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