目的：探索ATP柠檬酸裂解酶（ACLY）在结肠癌组织中的表达情况及其临床病理学意义，为结肠癌的诊治提供新思路。方法：以50例结肠癌患者为研究对象，用免疫组织化学染色方法观察结肠癌患者的癌组织及对应的癌旁组织标本中ACLY的表达，并研究ACLY表达的临床病理学意义。结果：在结肠癌组织中ACLY表达显著高于对应的癌旁组织（P=0）；ACLY高表达与结肠癌的浸润深度、淋巴结转移及TNM分期显著相关（P值分别为0、0.005及0.007）；与年龄、性别、肿瘤大小、组织分化程度无相关性（P>0.05）。结论：ACLY在结肠腺癌组织中高表达，且与肿瘤转移相关；ACLY有望成为结肠癌诊治的一个新靶点。

[1] KOCEMBA K A,DULINSKA-LITEWKA J,WOJDYLA K L,et al.The role of 6-phosphofructo-2-kinase (PFK-2)/fructose 2,6-bisphosphatase (FBPase-2)in metabolicreprogramming of cancercells[J].Postepy Hig Med Dosw,2016,70:938-950.
[2] PENKERT J,RIPPERGER T,SCHIECK M,et al.On metabolic reprogramming and tumor biology:A comprehensive survey of metabolism in breast cancer[J].Oncotarget,2016,7(41):67626-67649.
[3] MASOUDI-NEJAD A,ASGARI Y.Metabolic cancer biology:structural-based analysis of cancer as a metabolic disease,new sights and opportunities for diseasetreatment[J].Semin Cancer Biol,2015,30(2):21-29.
[4] 郑杰.肿瘤生物合成的异常及其临床应用[J].肿瘤,2012,32(1):74-78.
[5] OOKHTENS M,KANNAN R,LYON I,et al.Liver and adipose tissue contributions to newly formed fatty acids in an ascites tumor[J].Am J Physhol,1984,247(1):146-153.
[6] SANCHEZ-SOLANA B,LI D Q,KUMAR R.Cytosolic functions of MORC2 in lipogenesis and adipogenesis[J].Biochim Biophys Acta,2013,1843(2):316-326.
[7] 程桂丹,陈洪,陆枫林.肿瘤相关基因RASAL1在结肠癌中的表达及临床意义[J].世界华人消化杂志,2011,19(5):488-493.
[8] FROMOWITZ F B,VIOLA M V,CHAO S,et al.Ras p21 expression in the progressionof breast cancer[J].Hum Pathol,1987,18(12):1268-1275.
[9] 史悦,袁高峰,石凤灵,等.RAS、CD68和CD34在乳腺癌组织中的表达及其临床意义[J].临床肿瘤学杂志,2015,20(12):1080-1083.
[10] BAUER D E,HATZIVASSILIOU G,ZHAO F,et al.ATP citrate lyase is an important component of cell growth and transformation[J].Oncogene,2005,24(41):6314-6322.
[11] SATO R,OKAMOTO A,INOUE J,et al.Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins[J].J Biol Chem,2000,275(17):12497-12502.
[12] MIGITA T,NARITA T,NOMURA K,et al.ATP citrate lyase:activation and therapeutic implications in non-small cell lung cancer[J].Cancer Res,2008,68(20):8547-8554.
[13] 王丽影,雷群英,戴振宇.N-乙酰氨基葡萄糖转移酶过Ⅴ表达对人肝癌细胞粘着斑复合物介导的信号转导作用[J].中国生物化学与分子生物学报,2002,18(2):240-244.
[14] LORD K A,WANG X M,SIMMONS S J,et al.Variant cDNA sequences of human ATP:citrate lyase:cloning,expression,and purification from baculovirus-infected insect cells[J].Protein Expr Purif,1997,9(1):133-141.
[15] WAGNER P D,VU N D.Phosphorylation of ATP-citrate lyase by nucleoside diphosphate kinase[J].J Biol Chem,1995,270(37):21758-21764.
[16] PIERCE M W,PALMER J L,KEUTMANN H T,et al.ATP-citrate lyase structure of a tryptic peptide containing the phosphorylation site directed by glucagon and the cAMP-dependent protein kinase[J].J Biol Chem,1981,256(17):8867-8870.
[17] FUKUDA H,KATSEURADA A,IRITANI N.Effects of nutrients and hormones on gene expression of ATP citrate-lyase in rat liver[J].Eur J Biochem,1992,209(1):217-222.
[18] CHRISTENSEN L L,TTUE K,HAMILTON M P,et al.SNHG16 is regulated by the Wnt pathway in colorectal cancer and affects genes involved in lipid metabolism[J].Mol Oncol,2016,10(8):1266-1282.
[19] WANG Y,ZHOU C,SHI D,et al.Unfavorable effect of small tumor size on cause-specific survival in stage ⅡA colon cancer,a SEER-based study[J].Int J Colorectal Dis,2015,30(1):131-137.
[20] KORNPRAT P,POLLHEIMER M J,LINDTNER R A,et al.Value of tumor size as a prognostic variable in colorectal cancer:a critical reappraisal[J].Am J Clin Oncol,2011,34(1):43-49.