丹参酮IIA对成年大鼠睾丸扭转的保护作用和机制研究-东南大学学报医学版

丹参酮IIA对成年大鼠睾丸扭转的保护作用和机制研究

单位：1. 东南大学附属中大医院泌尿外科, 江苏南京 210009;
2. 青岛大学附属医院急诊科, 山东青岛 266000

分类号：R697+.2

出版年·卷·期（页码）：2017·36·第五期（795-799）

摘要：

目的：观察丹参酮ⅡA对成年大鼠睾丸扭转复位睾丸损伤的保护作用并初步探讨其机制。方法：对建立左侧睾丸扭转复位动物模型，将36只7周龄健康SD雄性大鼠随机分为3组：假手术组、生理盐水组、丹参酮ⅡA组，每组12只；分别于术后72小时、4周两批处死大鼠，每批每组6只，取左侧睾丸，行HE染色及组织损伤评分，Tunel染色，以及睾丸组织内Bcl-2蛋白检测（免疫印迹法），检测总活性氧（ROS），超氧化物歧化酶（SOD）、与丙二醛（MDA）含量，并测定大鼠血清睾酮水平。结果：与生理盐水组比较，丹参酮Ⅱ给药组在术后72小时睾丸组织损伤明显减轻，细胞凋亡减少，Bcl-2表达升高；在术后4周发现给药组SOD、大鼠血清睾酮水平明显上升，而组织内ROS和MDA水平含量下降，差异均具有显著性（P<0.05）。结论：单次腹腔内注射丹参酮ⅡA对成年大鼠单侧睾丸扭转损伤具有保护作用，其机制在早期可能是通过抗凋亡作用，而在远期则可能发挥了抗氧化作用。

Objective:To observe the protective effect of tanshinone ⅡA on testicular torsion and return to testicular injury in adult rats and to explore its mechanism. Methods:36 male SD rats aged 7 weeks were randomly divided into 3 groups:sham operation group, saline group and tanshinone ⅡA group, 12 rats in each group. The rats were sacrificed at 72 hours and 4 weeks after operation. Six rats in each group and left testes were subjected to HE staining and histological injury score, Tunel staining, and Bcl-2 protein test (immunoblotting), reactive oxygen (ROS), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured. Serum testosterone levels were measured as well. Results:Compared with the saline group, the testis tissue injury was significantly reduced in the tanshinone ⅡA group, the apoptotic cells were decreased and the expression of Bcl-2 was increased. The levels of SOD and the level of serum testosterone in rats was significantly higher than that in saline group (P<0.05). Conclusion:Single intraperitoneal injection of tanshinone Ⅱ has protective effect on unilateral testicular torsion injury in adult rats, possibly through the anti-apoptotic effect in early stage, and may play an antioxidant role in the long term.

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