Objective:To investigate the effects of the calcifying nanoparticles (CNPs) to establish a rat model of kidney stones on the levels of urine neutrophil gelatinase associated lipid carrier pale(NGAL), white light monocyte chemoattractant protein -1 (MCP-1) and kidney function. Methods:24 male SD SPF rats were randomly divided into experimental group and control group with 12 rats in each group, the experimental group adopted intravenous injection of CNPs to build rat renal calculus model, the control group was injected with saline. The level of MCP-1 and NGAL in rat urine, stone formation in rat kidney, pathological changes and the changes of renal function were measured at different time points. Results:The urine NGAL and MCP-1 levels in the experimental group after modeling 4h, 12h, 24h, 1 week, 2 weeks and 8 weeks showed increasing tendency, and the urine NGAL levels at each time point were higher than that in control group (P<0.05), after modeling 1 week, 2 weeks and 8 weeks the urine MCP-1 levels were higher than those of control group (P<0.05). The level of serum creatinine, urea nitrogen in rats of the experimental group in 8 weeks after surgery were higher than that in control group (P<0.05). Renal biopsy were found in the formation of stone crystals in the experimental group, the control group did not find the crystal stones form. Conclusion:The mechanism of CNPs to establish rat kidney stones model may be related to the increase of NGAL and MCP-1 levels, and CNPs can cause kidney functional damage. |
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