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利拉鲁肽激活Akt通路改善乳鼠心肌细胞缺氧/复氧损伤
作者:张文娟  周雪  李瑞雪  杨茜  夏丽芳  李志红 
单位:保定市第一中心医院 内分泌二科, 河北 保定 071000
关键词:利拉鲁肽 缺氧/复氧损伤 Akt通路 细胞凋亡 乳鼠 
分类号:R542.22;R33-33
出版年·卷·期(页码):2017·36·第五期(764-768)
摘要:

目的:研究利拉鲁肽对乳鼠心肌细胞缺氧/复氧损伤的影响及机制。方法:分离乳鼠心肌细胞,分为对照组、缺氧/复氧组和利拉鲁肽组,体外构建缺氧/复氧损伤模型,使用流式细胞术检测细胞凋亡情况,Western blot检测凋亡相关蛋白caspase-3、Akt以及Akt磷酸化水平。结果:对乳鼠心肌细胞进行缺氧/复氧损伤处理后,流式细胞术检测发现,与对照组相比,缺氧/复氧损伤能明显诱导细胞凋亡,而利拉鲁肽则有抗凋亡作用;Western blot结果提示,缺氧/复氧损伤可诱导caspase-3表达,激活凋亡相关信号通路,促进细胞凋亡,而利拉鲁肽组细胞Akt磷酸化水平明显增加,caspase-3表达减少、活性降低。结论:利拉鲁肽可以通过增加Akt磷酸化以激活Akt通路,抑制caspase-3的表达,发挥抗凋亡作用,改善缺氧/复氧损伤。

Objective:To study the effects of liraglutide on hypoxia/reoxygenation injury in neonatal rat cardiomyocytes and the underlying mechanism. Methods:The cardiomyocytes were isolated from neonatal rats, and then divided into control group, hypoxia/reoxygenation group and liraglutide group. The model of hypoxia/reoxygenation injury was established in vitro. Apoptosis was detected by flow cytometry and apoptosis related proteins including caspase-3, Akt, and phospho-Akt were detected by Western blot. Results:Cardiomyocytes of neonatal rats were treated with hypoxia/reoxygenation injury, compared with the control group, results of flow cytometry showed that hypoxia/reoxygenation injury could induce apoptosis of neonatal rat cardiomyocytes, while liraglutide had anti-apoptosis effects. Western blot showed that the expression of caspase-3 was activated by the hypoxia/reoxygenation injury. While in the liraglutide group, the phosphorylation of Akt was increased, as a result, the expression and activation of caspase-3 was suppressed. Conlusion:Liraglutide could improve the hypoxia/reoxygenation injury by increasing Akt phosphorylation to activate the Akt pathway, suppress the expression of caspase-3, and harbour an anti-apoptotic effect.

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