Objective:To investigate the effect and possible mechanism of Shenque(CV8) moxibustion therapy on the mice with autoimmune thyroiditis. Methods:Experimental autoimmune thyroiditis(EAT) model was replicated on mice with high iodine intake and subcutaneous immunization of porcine thyroglobulin (PTg). The mice were randomly divided into blank group, EAT model group and treatment group. The thyroid stimulating hormone (TSH)concentrations and thyroglobulin antibody (TGAb)concentrations were detected by enzyme-linked immunosorbent assay (ELISA). The morphological changes of thyroids and local skin tissues around CV8 were observed by HE staining. The expression of cytokine transforming growth factor-β1 (TGF-β1) and transient receptor potential vanilloid subfamily member 1 (TRPV1) of thyroid tissues and local skin tissues around CV8 were detected by immunohistochemistry (IHC). Results:Comparing with the EAT model group, the mice from treatment group presented lighter congestion, swollen and inflammatory cells infiltration, degeneration and necrosis of follicular epithelial cells in thyroid tissues, along with normal production of thyroglobulin. The expression of TGF-β1 and TRPV1 in the local skin tissues around CV8 were enhanced significantly after treatment(P<0.05), however, there were no significant changes in the thyroid tissues(P>0.05). The serum TGAb concentrations of treatment group were significantly lower comparing with the EAT model group(P<0.05), however, there was no significant differences in the serum TSH concentrations among groups(P>0.05). Conclusion:Using Moxibustion therapy on CV8 can significantly ease the inflammation in the thyroid tissues of EAT mice. The up regulation of TGF-β1 and TRPV1 in the local skin tissues around CV8 after moxibustion therapy may suppress the immune response, moreover, causing a down regulation of thyroid autoantibody.