Objective:To explore the expression of SPOCK1 in lung cancer tissue and its effects on prolifetion, invasion and migration. Methods:Lung cancer cell lines A549, H3255 were divided into interfere group and control group (NC group) according to interfection method, the expression of SPOCK1 in lung cancer cell line A549 and H3255 were inhibited by siRNA, the proliferation of cells were measured by MTT assay, the colony formation capacity were detected by colony formation assay, the migration capacity were mearsured by wound heal assay, the cell invasion capacity were mearsured by Transwelll assay, the expression of mRNA and protein related to mTOR-s6K pathway were measured by RT-PCR and Western blotting. Results:In A549 and H3255 cell lines, the cell proliferation rate, number of colonies, number of invasion cells, wound healing rates in siSPOCK1 group were significantly less than those in NC group, the difference were statistically significant (P<0.05). In SPOCK1 knockdown A549 cells, the G0/G1 phases cell were 66.5% and 54.3% in siSPOCK1 and NC group, respectively, the difference between the two groups was statistically significant (P<0.05). And in H3255 cells, the G0/G1 phases cell were 70.2% and 55.7% in siSPOCK1 and NC group, respectively, the difference between the two groups was statistically significant (P<0.05). In A549 and H3255 cell linse, the expression of mTOR, s6K mRNA in siSPOCK1 group was statistically decreased when compared to NC group, the difference between the two groups was statistically significant (P<0.05).The expression of p-mTOR, p-s6K protein in siSPOCK1 group were statistically decreased when compared to NC group, the difference between the two groups was statistically significant (P<0.05). Conclusion:Inhibition the expression of SPOCK1 can reduce the proliferation, invasion and migration capability of lung cancer cells, and its mechanism may be related to mTOR-s6K signaling pathway. |
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