Objective:To explore the effect of semaphorin 3A (Sema 3A) signaling pathway on invasion and proliferation of lung cancer cells induced by transforming growth factor -β1 (TGF-β1) and its mechanisms. Methods:The lung cancer A549 cells were divided into three groups:blank control group (control), TGF-β1 induced group (TGF-β1), 3A Sema pretreatment group (Sema 3A+TGF-β1). The control group was cultured with normal cultured, 5 μg·L-1 TGF-β1 was added in TGF-β1 group, 10 μmol·L-1 of Sema 3A was firstly added and pretreated for 40-50 min and then 5 μg·L-1 of TGF-β1 added in the Sema 3A+TGF-β1 group. Cell proliferation, invasion ability and the expression level of E-cadherin, Akt, P-Akt protein was measured. Results:After Sema 3A pretreatment, the Sema 3A+TGF-β1 group cells were round or oval. Sema 3A mRNA relative expression level in Sema 3A+TGF-β1 group were significantly higher than that in the control group and the TGF-β1 group(P<0.05). In 36-72 h, the proliferation rate of TGF-β1 group was significantly higher than in Sema 3A+TGF-β1 group and the control group(P<0.05), the proliferation rate in Sema 3A+TGF-β1 group was significantly higher than that in the control group(P<0.05). The migrated cells number was significantly higher than that in Sema 3A+TGF-β1 group and the control group(P<0.05),migrated cell number in Sema 3A+TGF-β1 group was significantly higher than that in the control group(P<0.05). The expression of E-cadherin protein was significantly lower than that in Sema 3A+TGF-β1 group and the control group, P-Akt protein was significantly higher than that in Sema 3A+TGF-β1 group and the control group(P<0.05). E-cadherin protein in Sema 3A+TGF-β1 group was significantly lower than that in the control group, P-Akt protein was significantly higher than that in the control group(P<0.05). Conclusion:Sema 3A can inhibit invasion and proliferation induced by TGF-β1, the mechanism may be related to the inhibition of Akt phosphorylation and up regulation of E-cadherin expression.
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