川芎嗪对人肝癌细胞TGF-β1/Smads通路的影响-东南大学学报医学版

川芎嗪对人肝癌细胞TGF-β1/Smads通路的影响

单位：贵州省人民医院肝胆外科, 贵州贵阳 550002

分类号：R282.710.7;R735.7

出版年·卷·期（页码）：2017·36·第四期（554-558）

摘要：

目的：研究川芎嗪（tetramethylpyrazine，TMP）对人肝癌细胞TGF-β1/Smads通路的影响，以探讨其抑制肝癌的原因。方法：将人肝癌细胞分为4个组，即对照组和不同质量浓度的TMP组（0.04、0.2、1.0 mg·ml^-1），各组给予对应药物干预24 h，CCK-8细胞计数法测定细胞增殖水平的变化，real-time PCR方法检测各组肝癌细胞TGF-β1、Smad4及Smad7 mRNA表达，Western blotting方法检测各组细胞TGF-β1及Smad4蛋白表达。结果：在TMP干预24 h后，肝癌细胞的增殖明显受到抑制，肝癌细胞TGF-β1及Smad7的mRNA及蛋白表达明显下调，而Smad4的mRNA及蛋白表达明显上调（均P<0.05），呈现浓度依赖性。结论：TMP的抗肝癌作用可能与它对肝癌细胞TGF-β1/Smads通路的调控有关。

Objective:To investigate tetramethylpyrazine(TMP) effects on transforming growth factor-β1 (TGF-β1)/Smads signaling pathway in hepatocellular carcinoma (HCC). Methods:HCC cells were divided into four groups:control group, different concentration of TMP(0.04,0.2 and 1.0 mg·ml^-1). After respectively incubated with 24 h, cells proliferation was investigated by CCK-8, Relative mRNA expression of TGF-β1, Smad4 and Smad7 in HCC were assessed by real-time PCR. Relative proteins expression of TGF-β1 and Smad4 were investigated by Western blotting. Results:TMP significantly attenuated cells proliferation, significantly down-regulated both the mRNA and protein expression of TGF-β1 and Smad7, significantly up-regulated the mRNA and protein expression of Smad4 (all P<0.05). Conclusion:TMP effects anti-HCC possibly through modulating TGF-β1/Smads signaling pathway.

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