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阿霉素增强CIK细胞抗多发性骨髓瘤细胞的机制研究
作者:姚阳1  周民2  卢绪章3  姜玉3  岑岭3  张修文3  杨建和3 
单位:1. 泰州职业技术学院, 江苏 泰州 225300;
2. 常州市第三人民医院, 江苏 常州 213000;
3. 南京医科大学附属常州第二人民医院, 江苏 常州 213000
关键词:NKG2D配体 细胞因子诱导的杀伤性细胞 骨髓瘤细胞 
分类号:R329.2
出版年·卷·期(页码):2017·36·第一期(20-23)
摘要:

目的:探讨阿霉素增强细胞因子诱导的杀伤性(CIK)细胞抗多发性骨髓瘤细胞的机制。方法:用阿霉素处理骨髓瘤细胞株U266以及患者骨髓瘤细胞,流式细胞仪(FCM)检测细胞表面NKG2D配体(MICA/B和ULBPs)表达的变化及CIK细胞对U266细胞杀伤作用的变化。结果:阿霉素处理后U266细胞表面MICA/B表达明显升高(P=0.002),而ULBPs的表达无明显变化。阿霉素亦能够诱导患者骨髓瘤细胞表面MICA/B的表达增加。CIK细胞对阿霉素处理过的U266细胞杀伤作用明显增加(P=0.01),这种杀伤作用可以被抗NKG2D抗体部分抑制(P=0.03)。结论:化疗药物诱导骨髓瘤细胞表面MICA/B表达的增加,从而增强CIK细胞对骨髓瘤细胞的杀伤作用。

Objective:To explore the mechanism of adriamycin enhanced CIK cells cytotoxicity against multiple myeloma cells.Methods:Multiple myeloma cell line U266 and the cells from patients with multiple myeloma were treated with adriamycin,the expression of NKG2D ligands (MICA/B and ULBPs) were analyzed by flow cytometry (FCM).The cytotoxicity of CIK cells against U266 cell was detected by flow cytometry.Results:The expression of MICA/B on multiple myeloma cell line U266 was up-regulated after treated with adriamycin (P=0.002),however expression of ULBPs had no changed.The expression of MICA/B on primary plasma cells was also up-regulated after treated with adrimycin.The cytotoxicity of CIK cell against U266 was significantly increased by treated with adriamycin (P=0.01),and the enhancing cytotoxicity was partly blocked by anti-NKG2D Abs (P=0.03).Conclusion:The cytotoxicity of CIK cells against multiple myeloma an enhance by treated with adriamycin through Up-regulating of MICA/B.

参考文献:

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