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长链非编码RNA CCAT1对前列腺癌PC-3细胞增殖、迁移及凋亡的影响
作者:韩勖1  凌志新1  葛增乐1  胡强1  许斌2  陈明2 
单位:1. 东南大学 医学院, 江苏 南京 210009;
2. 东南大学附属中大医院 泌尿外科, 江苏 南京 210009
关键词:结肠癌相关转录因子1 前列腺癌 增殖 迁移 凋亡 
分类号:R737.25
出版年·卷·期(页码):2017·36·第一期(1-4)
摘要:

目的:探讨长链非编码RNA结肠癌相关转录因子1(CCAT1)对前列腺癌PC-3细胞增殖、迁移及凋亡的影响。方法:采用荧光定量PCR检测CCAT1 siRNA在PC-3细胞中的敲低效率;通过CCK-8法、transwell迁移实验、流式细胞仪(FACS)检测低表达CCAT1对PC-3细胞增殖、迁移及凋亡的影响。结果:PC-3细胞转染CCAT1 siRNA后,qPCR检测细胞中CCAT1表达量明显降低;低表达CCAT1能显著抑制细胞增殖,减弱迁移能力,促进细胞凋亡。结论:CCAT1可能通过对细胞增殖、迁移及凋亡的调控促进前列腺癌发生、发展。

Objective:To explore the effect of IncRNA colon cancer associated transcript 1(CCAT1) on the proliferation,migration and apoptosis of prostate cancer PC-3 cells.Methods:qPCR assay was performed to confirm the low expression level of CCAT1 after the transfection of CCAT1 siRNA.CCK-8 assay,transwell migration assay and flow cytometry (FACS) were used to observe on its role in cell proliferation,migration and apoptosis.Results:The expression level of CCAT1 was efficiently knocked down with the transfection of CCAT1 siRNA.And knockdown CCAT1 could significantly suppress the ability of proliferation,migration and promote apoptosis.Conclusion:CCAT1 may promote the carcinogenesis of prostate cancer through regulation of cell proliferation,migration and apoptosis.

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