新疆维吾尔族宫颈癌患者人乳头瘤病毒感染与外周血中Th17细胞水平的相关性研究-东南大学学报医学版

新疆维吾尔族宫颈癌患者人乳头瘤病毒感染与外周血中Th17细胞水平的相关性研究

单位：1. 新疆医科大学附属肿瘤医院肿瘤防治研究所, 新疆乌鲁木齐 830011;
2. 新疆医科大学附属肿瘤医院检验科, 新疆乌鲁木齐 830011

分类号：R737.33

出版年·卷·期（页码）：2016·35·第五期（699-703）

摘要：

目的：检测新疆维吾尔族宫颈癌患者人乳头瘤病毒（HPV）感染状况及外周血中Th17细胞比例，探讨HPV感染与Th17细胞表达的关系。方法：采用二代杂交捕获法及流式细胞术分别检测宫颈癌组（维吾尔族50例）、正常对照组（维吾尔族50例）的HPV感染状况和外周血中Th17细胞比例。结果：维吾尔族宫颈癌组HPV检出率96.0%，正常对照组HPV检出率为6.0%，两组比较差异有统计学意义（P<0.05）；不同期别的宫颈癌的HPV病毒载量差异有统计学意义（P<0.05），且随宫颈癌的FIGO分期期别越高，HPV病毒载量越高。维吾尔族宫颈癌组患者外周血Th17细胞占淋巴细胞的比例较正常对照组明显升高，差异有统计学意义（P<0.05）。Th17细胞水平在HPV阳性与阴性组之间比较，差异有统计学意义（P<0.05），并且随HPV载量的升高，Th17细胞表达水平也升高，两者呈正相关（r=0.55，P<0.001）；外周血Th17细胞表达水平与FIGO分期、是否有淋巴结转移、脉管浸润均呈正相关（r值分别为0.75、0.59、0.62，均P<0.001）。结论：Th17细胞在HPV持续感染所致宫颈癌的发生发展中可能起着重要作用。

Objective: To investigate the correlation between human papilloma virus(HPV) and Th17 cells in peripheral blood of Uygur patients with cervical cancer. Methods: Peripheral blood was collected from 50 cases of Uygur cervical cancer patients and 50 Uygur healthy people.Hybrid capture Ⅱ method was used to detect viral load of HPV.The percentage of Th17 cells was detected by flow cytometric analysis. Results: The rate of HR-HPV infection was 96.0% in the cervical cancer group,6.0% in healthy control group(P<0.05).The percentages of Th17 cells in cervical cancer group was significantly higher than that in the healthy control group(P<0.05).The percentages of Th17 cells between HPV positive and negative group was statistically significantly different(P<0.05).The expression of Th17 cells in peripheral blood was positively correlated with FIGO stage,lymph node metastasis,and vascular infiltration(r=0.55,0.59,0.62,P<0.001). Conclusion: Th17 cells may play an important role in the development of persistent HPV infection leading to cervical cancer.

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