吡那地尔对术后持续性疼痛大鼠脊髓JNK/MCP-1释放的影响-东南大学学报医学版

吡那地尔对术后持续性疼痛大鼠脊髓JNK/MCP-1释放的影响

单位：1. 苏州大学附属第一医院麻醉科, 江苏苏州 215006;
2. 南通大学附属医院麻醉科, 江苏南通 226001

分类号：R-332;R972.4

出版年·卷·期（页码）：2016·35·第五期（678-682）

摘要：

目的：观察吡那地尔（pinacidil）对术后持续性疼痛大鼠脊髓JNK/MCP-1释放的影响。方法：随机将35只大鼠分为正常组（Control组）、假手术组（Sham组）、切割+牵拉皮肤肌肉组（SMIR组）、溶剂+SMIR组（Vehicle组）、吡那地尔+SMIR（Pina组）、吡那地尔+格列苯脲+SMIR组（Pina+Gli组）、格列苯脲+SMIR组（Gli组）等7组。检测各组机械刺激缩足反射痛阈值（MWT），Western blot/ELISA法检测脊髓JNK/MCP-1蛋白表达。结果：（1）与Sham组相比，SMIR组MWT在术后3 d开始降低（P<0.01），并持续21 d（P<0.001）以上；（2）SMIR术后持续显著上调脊髓JNK/MCP-1的表达（P<0.01）；（3）鞘内注射吡那地尔显著下调术后SMIR诱导的脊髓JNK/MCP-1表达（P<0.01）和上调MWT（P<0.01），格列苯脲能阻断吡那地尔所引起的上述变化（P<0.05）。结论：吡那地尔能抑制切割及牵拉皮肤肌肉所致的术后持续性疼痛，其机制可能与抑制JNK/MCP-1的上调有关。

Objective:To observe the effect of pinacidil on JNK/MCP-1 expression in spinal cord of a rat model of persistent postoperative pain. Methods:SD rats were divided into 7 groups randomly:control group(Control group), sham operation group (Sham group), skin/muscle incision and retraction group (SMIR group), vehicle+SMIR group (Vehicle group), pinacidil+SMIR (Pina group), pinacidil+ glibenclamide+SMIR group (Pina+Gli group) and glibenclamide+SMIR group (Gli group).The mechanical withdrawal threshold (MWT) of the left hind paw of rats in different group was measured at different time points after the surgery. Western blot/ELISA were used to measure the protein expression level of JNK/MCP-1. Results:(1) Compared with Sham group, MWT in SMIR group was reduced significantly from 3 d after the operation (P<0.01), and lasted for more than 21 d (P<0.001). (2) The protein expression of JNK/MCP-1 were significantly increased after SMIR as compared with the control group(P<0.01). (3) Intrathecal injection of pinacidil attenuated the express of JNK/MCP-1 in the spinal cord (P<0.01) and up-regulated the MWT (P<0.01), which could be blocked by glibenclamide (P<0.05). Conclusion:Pinacidil inhibits the persistent postoperative pain caused by SMIR. The mechanisms may be partly due to the inhibition of JNK/MCP-1 upregulation.

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