Slug与食管癌侵袭转移间的关系及其调控机制研究-东南大学学报医学版

Slug与食管癌侵袭转移间的关系及其调控机制研究

单位：1. 邯郸市第一医院, 河北邯郸 056002;
2. 磁县人民医院, 河北邯郸 056500

分类号：R735.1

出版年·卷·期（页码）：2016·35·第四期（569-574）

摘要：

目的：研究Slug与食管癌侵袭转移间的关系及其调控机制。方法：分别将构建好的pcDNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中，以pcDNA 3.1和Lip 2000空转作为对照组，显微镜下观察转染前后细胞的形态变化，检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化，另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果：转染pcDNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pcDNA 3.1-Slug的Eca-109中E-钙黏素表达下调，N-钙黏蛋白和波形蛋白表达上调，细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调，N-钙黏蛋白和波形蛋白表达下调，细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关（r=-0.96）。结论：转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力，而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。

Objective:To study the association of Slug with invasion and metastasis of esophageal carcinoma and its regulating mechanism. Methods:pcDNA 3.1-Slug recombinant plasmid was constructed and transfected into esophageal carcinoma cell lines of Eca-109, with pcDNA 3.1 and Lip 2000 mock transfection served as control group. The morphological changes of cells were observed under the microscope. The expressions of E-cadherin, N-cadherin and vimentin were determined, and invasion ability of the cells before and after transfection was detected by Transwell chamber method. Results:The cells of miR-140/Eca-109 and pcDNA 3.1-Slug/Eca-109 became elongated. The expression of E-cadherin was down-regulated, and N-cadherin and waveform protein was increased in the Eca-109 cells when transfected with pcDNA 3.1-Slug.The expression of E-cadherin was up-regulated, and N-cadherin and waveform protein was decreased in the Eca-109 cells when transfected with miR-140.The number of cells through the Matrigel was significantly reduced. The expression of miR-140 and Slug in esophageal carcinoma cells was negatively correlated(r=-0.96). Conclusion:The Slug transcription factor can improve the ability of mesenchymal transition and invasion of esophageal epithelial cells, and miR-140 may regulate the cell invasion of Eca-109 via controlling Slug expression.

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