自发性高血压大鼠心肌组织Sirt3表达的增龄性变化-东南大学学报医学版

自发性高血压大鼠心肌组织Sirt3表达的增龄性变化

单位：1. 西安交通大学医学院第二附属医院心内科, 陕西西安 710004;
2. 西安交通大学医学院第二附属医院病理科, 陕西西安 710004;
3. 西安交通大学医学院第二附属医院脑病科, 陕西西安 710004

分类号：R-33;R544.1

出版年·卷·期（页码）：2016·35·第四期（487-491）

摘要：

目的：探讨自发性高血压（SH）大鼠心肌组织沉默信息调节因子3（Sirt3）表达的增龄性变化。方法：将36只不同周龄Wistar-Kyoto（WKY）大鼠和SH大鼠分为WKY 16、32、64周3个周龄组和SH 16、32、64周3个周龄组，每组6只。采用尾套测压法测量各组大鼠尾动脉收缩压，采用Masson染色观察心肌纤维化，免疫组化染色及免疫组织印迹法检测左心室心肌组织Sirt3的表达。结果：与同周龄WKY大鼠相比，SH大鼠血压显著升高（P<0.05），心肌组织胶原纤维组织明显增多，Sirt3蛋白表达水平均显著下降（均P<0.05），且呈增龄性下降趋势。结论：SH大鼠肥厚的心肌组织sirt3表达呈增龄性下降。

Objective:To investigate the expression changes of Sirt3 with age in left ventricle of spontaneously hypertensive (SH) rats. Methods:Thirty-six Wistar-Kyoto (WKY) and SH rats of different age were divided into WKY (16, 32, 64 weeks) and SHR (16, 32, 64 weeks) groups with 6 rats in each group. Systolic blood pressure of rats was measured by tail cuff method and left ventricular fibrosis was detected by Masson staining. The level of Sirt3 expression was analyzed by immunohistochemistry staining and Western blotting. Results:In comparison with WKY rats of the same age, the systolic blood pressure (P<0.05) and left ventricular fibrosis of rats in SH rats were higher. The expression of Sirt3 protein was lower in the SH rat group and was decreased with age (P<0.05).Conclusion:Sirt3 expression in the hypertrophic left ventricle of SH rats is decreased with age.

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