>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
胃黏膜组织TNF-α及iNOS表达水平与胃炎发病及细胞凋亡的相关性研究
作者:陈东 
单位:长沙市第一医院 急诊科, 湖南 长沙 410005
关键词:肿瘤坏死因子α 诱导型一氧化氮合酶 胃炎 细胞凋亡 
分类号:R573.3;R329.25
出版年·卷·期(页码):2016·35·第三期(370-373)
摘要:

Objective:To study the relationship of the expression of TNF-α, inducible nitric oxide synthase(iNOS) and with apoptosis and gastritis. Methods:112 patients with gastritis as a study group and 56 healthy volunteers of the same age as a control group were selected. All patients' liver function, blood routine, blood pressure and heart rate were examined the next day in the hospital. Next, the study group was furthermore divided into 3 groups according to illness severity, the levels of TNF-α and iNOS were compared. Apoptosis was detected by TUNEL assay. Finally, Pearson correlation analysis methods were used to analyze the correlation on iNOS, TNF-α with gastritis and apoptosis. Results:(1) The more severe the disease, the higher the levels of iNOS and TNF-α would be. Moreover, both iNOS and TNF-α levels were significantly higher than those in the control group. (2) Apoptotic cells were significantly different from normal cells in morphology, size and TUNEL staining. At the same time, when TNF-α ≥1.0 ng·mg-1, AI was significantly higher than that when TNF-α<1.0 ng·mg-1. When iNOS≥100 mm-2, AI was significantly higher than that when iNOS<100 mm-2. (3) Both iNOS and TNF-α levels positively correlated with the incidence of gastritis and apoptosis with significant differences. Conclusion:Inflammatory reaction of gastric mucosa for patients with gastritis may be related to the secretion of TNF-α and iNOS and they may induce apoptosis of gastric mucosal cells.

参考文献:

[1] 游海梅.IL-10、TNF-α在不同菌型幽门螺杆菌感染消化性溃疡中的表达[D].南昌:南昌大学,2014.
[2] 张志广.Hp感染胃癌组织中HIF-1α和iNOS的表达及相关性研究[D].天津:天津医科大学,2009.
[3] 王玉喆.二苯乙烯苷口服液稳定性及其对LPS诱导的小胶质细胞iNOS表达调控的研究[D].南通:南通大学,2013.
[4] 贺龙风.高危型HPV感染的宫颈病变组织中HIF-1α、iNOS的表达和相关性研究[D].天津:天津医科大学,2012.
[5] 成映霞,段永强,朱立鸣,等.益气健脾活血中药对萎缩性胃炎TNF-α、IL-2和EGF表达的影响[J].中国老年学杂志,2013,15:3649-3651.
[6] 张小艳,王曙升,宋瑛,等.IL-10、iNOS在幽门螺旋杆菌相关性胃炎及胃癌前病变组织中表达的相关性研究[J].现代肿瘤医学,2013,21(8):1830-1833.
[7] 杨菲.胃癌发生的高危因素及其与TNF-α的相关性研究[D].大连:大连医科大学,2012.
[8] 杜安杰.氨基胍对ROP视网膜神经细胞凋亡及iNOS表达的影响[D].石河子:石河子大学,2013.
[9] 孙波.肿瘤坏死因子α和白介素1β及诱导型一氧化氮合酶的表达与幽门螺杆菌相关性胃炎的关系[J].中国全科医学,2010,11:1155-1157.
[10] 江燕,宋澄跃,王桂周,等.幽门螺杆菌阳性的慢性胃炎患者胃黏膜组织中TNF-α、IL-1β、iNOS的表达[J].蚌埠医学院学报,2012,37(5):556-558.
[11] 尹姣.灭幽汤对幽门螺杆菌感染小鼠胃粘膜组织NF-κBp65、TLR2表达的影响[D].长沙:湖南中医药大学,2014.
[12] UEFUJI K,ICHIKURA T,MOCHIZUKI H,et al.Expression of cyclooxygenase-2 in human gastric adenomas and adenocarcinomas[J].J Surg Oncol,2001,76(1):26-30.
[13] 赵继亭.iNOS在NSAIDs相关性胃病患者胃粘膜中的表达及中药干预研究[D].济南:山东中医药大学,2014.
[14] SAUKKONEN K,NIEMINEN O,van REES B,et al.Expression of cyclooxygenase-2 in dysplasia of the stomach and in intestinal-type gastric adenocarcinoma[J].Clin Cancer Res, 2001,7(7):1923-1931.
[15] FOSSLIEN E.Molecular pathdogy of cyclooxygenase-2 in neoplasia[J].Ann Clin Lab,2000,30(1):3-21.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 405593 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-83272481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364