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LSD1和KDM5家族在卵巢癌细胞中的表达及LSD1对细胞增殖迁移的影响
作者:贾超1 2  王洁3  邵根宝3  陆祖宏1 
单位:1. 东南大学生物科学与医学工程学院 生物电子学国家重点实验室, 江苏 南京 210096;
2. 南京农业大学 动物科技学院, 江苏 南京 210095;
3. 江苏大学 医学院, 江苏 镇江 212013
关键词:赖氨酸特异去甲基化酶 卵巢癌 细胞增殖 细胞迁移 
分类号:R737.31;R73-3
出版年·卷·期(页码):2016·35·第三期(350-354)
摘要:

目的:研究赖氨酸特异去甲基化酶1(LSD1)和赖氨酸去甲基化酶5家族(KDM5家族成员)在人卵巢癌细胞中的表达,分析LSD1对细胞增殖迁移的影响。方法:在3种卵巢癌细胞株中,分别用RT-PCR检测LSD1、KDM5A、KDM5B、KDM5C、KDM5D的mRNA的表达,蛋白质印迹法检测前4种酶的表达;在人正常卵巢上皮细胞和癌细胞株中,采用蛋白质印迹法检测LSD1的表达;用MTT法检测LSD1对卵巢癌细胞株增殖的影响;应用划痕试验检测LSD1对卵巢癌细胞株迁移的影响。结果:在3种人卵巢癌细胞的上述5种酶中,LSD1表达水平最高(P<0.01),KDM5家族成员在不同细胞系中差异表达。LSD1抑制剂反苯环丙胺处理卵巢癌细胞后,用MTT法发现抑制LSD1能显著降低癌细胞增殖(P<0.05),刮痕实验发现该药物可显著抑制卵巢癌细胞的迁移(P<0.05)。结论:LSD1在卵巢癌中高表达,KDM5家族差异化表达;LSD1与卵巢癌细胞系的增殖和迁移能力密切相关。

Objective:To investigate the expression of lysine-specific demethylase 1(LSD1) and lysine demethylase 5 (KDM5), as well as the role of LSD1 on proliferation and migration within human ovarian cancer cells. Methods:Relative mRNA and protein levels of LSD1, KDM5A, KDM5B, KDM5C, KDM5D were detected respectively by real-time quantitative and Western blot in SKOV-3, HO8910, 3AO cells. The expressions of LSD1 were comparatively analyzed between a human ovarian epithelial cell line and three ovarian cancer cell lines by Western blot. In addition, the effects of LSD1 on proliferation and migration were determinted by MTT and wound-healing assay respectively. Results:The expression of LSD1 was highest(P<0.01), and the expression of KDM5 members was different in these ovarian cancer cells. Tranylcypromine, the inhibitor of LSD1, significantly decreased the proliferation and migration(P<0.05). Conclusion:LSD1 expresse at high level and the member of KDM5 family expresse at differentiated level within 3 ovarian cancer cell lines, and LSD1 is significantly correlated with the proliferation and migration within these 3 ovarian cancer cell lines.

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