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长链非编码RNA RP11-191L9.4促进前列腺癌PC-3细胞的迁移和侵袭
作者:王京1  黄烨清1  许斌2  陈明2 
单位:1. 东南大学 医学院, 江苏 南京 210009;
2. 东南大学 附属中大医院, 江苏 南京 210009
关键词:lncRNA RP11-191L9.4 前列腺癌 细胞增殖 细胞迁移 肿瘤侵袭 
分类号:R737.25;R-33;R329.25
出版年·卷·期(页码):2016·35·第三期(305-310)
摘要:

目的:探讨长链非编码RNA RP11-191L9.4对前列腺癌PC-3细胞增殖、迁移及侵袭能力的影响。方法:荧光定量PCR检测RP11-191L9.4 siRNA在PC-3细胞中的敲除效率; 通过MTT实验和克隆形成实验观察在PC-3细胞中低表达RP11-191L9.4对其增殖的影响;通过transwell细胞迁移实验检测敲低RP11-191L9.4的表达对PC-3细胞的迁移能力影响;通过Matrigel侵袭实验检测敲低RP11-191L9.4的表达对PC-3细胞侵袭能力的影响。结果:向前列腺癌细胞系PC-3中转染RP11-191L9.4 siRNA 48 h后,qPCR检测PC-3细胞中RP11-191L9.4明显低表达;MTT及克隆形成实验结果显示,敲低RP11-191L9.4的表达能显著抑制PC-3细胞的增殖;transwell迁移实验结果显示,敲低RP11-191L9.4的表达能减弱PC-3细胞的迁移能力;Matrigel胶细胞侵袭实验结果显示,敲低RP11-191L9.4的表达能减弱PC-3细胞的侵袭能力。结论:转染RP11-191L9.4 siRNA能显著抑制PC-3细胞中RP11-191L9.4的表达,而敲低RP11-191L9.4的表达可显著抑制前列腺癌细胞PC-3增殖、迁移及侵袭能力。

Objective:To explore the effect of lncRNA RP11-191L9.4 on the proliferation, migration and invasion ability of prostate cancer PC-3 cells.Methods:qPCR assay was used to confirm the low expression level of RP11-191L9.4 after the transfection of RP11-191L9.4 siRNA.MTT assay and colony-forming unit assay were performed to evaluate the effect of RP11-191L9.4 knock-down on PC-3 cell proliferation. Transwell migration assay was used to evaluate the effect of RP11-191L9.4 knock-down on the migration ability of PC-3 cells. Matrigel invasion assay was used to evaluate the effect of RP11-191L9.4 knock-down on the invasion ability of PC-3 cells. Results:The expression level of RP11-191L9.4 was significantly knocked down by the transfection of siRNA.MTT assay and colony forming unit assay showed that reducing the expression of RP11-191L9.4 can significantly weaken the proliferation ability of PC-3 cells. The result of transwell migration assay indicated that reducing the expression of RP11-191L9.4 in PC-3 cells obviously suppressed its migration ability. The result of Matrigel invasion assay demonstrated that reducing the expression of RP11-191L9.4 significantly suppressed the invasion ability of PC-3 cells. Conclusion:RP11-191L9.4 level in PC-3 cells can be efficiently knocked down with the transfection of RP11-191L9.4 siRNA, and knock down the expression level of RP11-191L9.4 can significantly suppress the ability of proliferation, migration and invasion in PC-3 cells.

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