乌司他丁对缺血再灌注大鼠脑保护及其机制研究-东南大学学报医学版

乌司他丁对缺血再灌注大鼠脑保护及其机制研究

单位：1. 四川省肿瘤医院手术麻醉科, 四川成都 610041;
2. 第三军医大学附属西南医院手术麻醉科, 重庆 400038

分类号：R453.9

出版年·卷·期（页码）：2016·35·第二期（199-201）

摘要：

目的:探讨乌司他丁对成年SD大鼠脑缺血再灌注损伤的保护作用及其机制。方法:将成年雄性SD大鼠随机分为空白对照组、假手术对照组、脑缺血再灌注损伤组、乌司他丁组,每组12只。空白对照组不做手术处理;假手术对照组麻醉后仅分离颈总动脉,20 min后缝合切口;脑缺血再灌注组麻醉后建立脑缺血再灌注损伤模型;乌司他丁组在脑缺血再灌注组的基础上于再灌注时静脉注射乌司他丁50μg·kg^-1。4组大鼠在手术后6、24、72 h取静脉血测Sl00β蛋白、神经烯醇化酶(NSE)含量。所有大鼠在术后72 h取血后断头处死,取脑组织测IL-1β、TNF-α含量。结果:与空白对照组比较,假手术对照组、脑缺血再灌注组、乌司他丁组术后6、24、72 h静脉血Sl00β蛋白、NSE表达均上升(P<0.01),术后72 h脑组织IL-1β、TNF-α表达均上升(P<0.01)。与假手术对照组比较,脑缺血再灌注组术后6、24、72 h Sl00β蛋白、NSE表达明显上升(P<0.01),术后72 h脑组织IL-1β、TNF-α表达均明显上升(P<0.01)。与脑缺血再灌注组比较,乌司他丁组术后6、24、72 h Sl00β蛋白、NSE表达明显下降(P<0.01);术后72 h脑组织IL-1β、TNF-α表达均明显下降(P<0.01)。结论:手术中脑缺血再灌注可导致大鼠神经细胞损伤,引发大脑炎症反应;乌司他丁可抑制这种损伤,降低大脑炎症反应。

Objective:To investigate the protective effect and the mechanism of ulinastatin on the brain ischemic-reperfusion injury in rats. Methods:Male Sprague-Dawley adult rats were randomly allocated to control group, surgery alone group, ischemic-reperfusion brain injury group, and ulinastatin group. Rats in surgery alone group received surgery without ischemic-reperfusion. Rats in ischemic-reperfusion brain surgery group received ischemic-reperfusion brain surgery. Rats in ulinastatin group received intravenous injection of ulinastatin(50μg·kg^-1) at the time after ischemic-reperfusion brain injury. The concentrations of S100β and NSE in plasma were measured by ELISA at 6, 24, 72 h after operation. Brain IL-1β and TNF-α were measured by ELISA at 72 h after operation. Results:Compared with control group, the expression of S100β and NSE were enhanced in the surgery alone group, ischemic-reperfusion brain injury group and ulinastatin group at 6, 24, 72 h after operation(P<0.01), and brain expression of IL-1β and TNF-α were enhanced at 72 h after operation(P<0.01). Compared with injury alone group, the expression of S100β and NSE were enhanced in ischemic-reperfusion brain surgery group and ulinastatin group at 6, 24, 72 h after operation(P<0.01) and brain expression of IL-1β, TNF-α were enhanced at 72 h after operation(P<0.01). Compared with ischemic-reperfusion brain injury group, the expression of S100β and NSE were diminished at 6, 24, 72 h after operation(P<0.01) and the expression of IL-1β and TNF-α were diminished at 72 h after operation in ulinastatin group(P<0.01). Conclusion:Ulinastatin can restrain inflammation in central nervous system and diminish the nervous impairment after ischemic-reperfusion brain injury in rats.

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