探讨溶血、乳糜血、自身抗体对HIV抗体初筛结果的影响-东南大学学报医学版

探讨溶血、乳糜血、自身抗体对HIV抗体初筛结果的影响

单位：1. 南京市职业病防治院检验科, 江苏南京 210004;
2. 南京市职业病防治院皮肤科, 江苏南京 210004

分类号：R512.91;R446.61

出版年·卷·期（页码）：2016·35·第一期（54-57）

摘要：

目的:探讨溶血、乳糜血、自身抗体阳性标本对酶联免疫吸附测定人类免疫缺陷病毒(enzyme linked immunosorbent assay-human immunodeficiency virus,ELISA-HIV)抗体初筛结果的影响。方法:对80份对照血样本、80份不同程度的溶血、240份不同程度的乳糜血以及80份自身抗体阳性血样本同时用两种ELISA检测试剂检测HIV抗体,并对ELISA-HIV抗体待复检的血样本用蛋白质印迹法(Western blotting,WB)进行复检。结果:80份溶血标本和240份乳糜血样本中,分别有3份样本ELISA-HIV抗体结果待复检,造成ELISA-HIV假阳性率分别为3.75%(3/80)和1.25%(3/240);80份自身抗体阳性血清样本检测后显示,4份样本HIV抗体结果待复检,它们分别来源于类风湿关节炎、系统性红斑狼疮、银屑病和特发性血小板减少性紫癜患者血清,自身抗体造成ELISA-HIV抗体假阳性率为5.00%(4/80)。结论:溶血、乳糜血、自身抗体对ELISA-HIV抗体初筛结果均有一定的影响,均可提高其假阳性率。

Objective:To explore the influences of hemolysis, chyle blood, autoantibodies on ELISA-HIV antibody screening results. Methods:80 control blood samples, 80 different degrees of hemolysis samples, 240 different levels of chyle blood samples and 80 autoantibody positive samples were detected by two kinds of ELISA-HIV antibody detection reagent, and the blood samples of ELISA-HIV antibody positive were reviewed by the Western blotting (WB) method. Results:There were 3 sample ELISA-HIV antibody screening test results were to be reviewed, respectively in 80 hemolysis samples and 240 chyle blood samples.ELISA-HIV false positive rate were 3.75%(3/80) and 1.25%(3/240),respectively. Within 80 serum samples of autoantibody positive 4 samples HIV antibody screening results were to be reviewed.They were rheumatoid arthritis(RA), systemic lupus erythematosus (SLE),Psoriasis(PsA) and idiopathic thrombocytopenic purpura(ITP),respectively.Autoantibodies cause HIV antibody screening test false positive rate was 5.00%(4/80). Conclusion:Hemolysis, chyle blood, autoantibodies have a certain influence on ELISA-HIV antibody screening test.They may lead to the false positive results in ELISA-HIV antibody screening test.

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