星形胶质细胞上调基因-1表达与食管鳞状细胞癌预后的相关性研究-东南大学学报医学版

星形胶质细胞上调基因-1表达与食管鳞状细胞癌预后的相关性研究

单位：1. 南京医科大学附属明基医院消化内科, 江苏南京 210019;
2. 南京医科大学第一附属医院消化内科, 江苏南京 210029

关键词：星形胶质细胞上调基因-1 食管鳞状细胞癌 RT-PCR Western boltting 免疫组织化学

分类号：R735.1

出版年·卷·期（页码）：2015·34·第六期（938-942）

摘要：

目的:探讨食管鳞状细胞癌组织中星形胶质细胞上调基因-1(AEG-1)的表达情况及其与临床分期、预后的相关性.方法:应用半定量RT-PCR、Western blotting 及免疫组织化学技术检测AEG-1基因在食管鳞状细胞癌组织中的表达情况,并与肿瘤临床分期特征及预后进行相关性分析.结果:食管鳞状细胞癌组织中AEG-1 mRNA及蛋白表达量分别为癌旁正常组织的8.2~18.5倍及4.6~13.2倍.76例食管鳞状细胞癌石蜡包埋组织中AEG-1 蛋白表达阳性占70例.AEG-1在男性患者中的表达强度强于女性患者,在中晚期病变中的表达强度强于早期病变,另AEG-1表达强度与患者生存时间呈负相关.结论:AEG-1不仅可以用于预测食管癌患者预后,也可能成为一个潜在的基因治疗靶点.

Objective: To investigate the expression of astrocyte elevated gene-1 (AEG-1) in esophageal squamous cell carcinoma tissue and its correlation with the clinical staging and prognosis. Methods: We used semi quantitative RT-PCR, Western blotting and immunohistochemistry to detect AEG-1 gene in esophageal squamous cell carcinoma tissue and different levels of expression, and analyze relationship with clinical tumor stage characteristics and prognosis. Results: The esophageal squamous cell cancer of AEG-1 mRNA and protein expression were 8.2-18.5 times and 4.6-13.2 times respectively those of adjacent normal tissues.Among 76 cases of esophageal squamous cell carcinoma AEG-1 in paraffin embedded tissue protein expressed in 70 cases. The expression intensity of AEG-1 was higher in male patients than female patients, as well as in advanced lesions than in early lesions, whereas its expression was negatively correlated with the survival time of the patients. Conclusion: Not only can the expression of AEG-1 in esophageal cancer predict the prognosis of patients, but can also be used as a potential molecular target for gene therapy.

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