永生化细胞是一种在理化刺激、基因突变、病毒感染等外界刺激下,可以在体外无限增殖的不衰老细胞株。细胞永生化机制有放射性因素、端粒-端粒酶激活、病毒基因转染及抑癌基因受抑制等学说。作者在总结细胞永生化原理的基础上阐述不同组织细胞永生化的技术方法及生长状态。

[1] OBINATA M.Immortalized cell lines with differentiation potentials:their establishment and possible application[J].Seikagaku,2008,80(1):5-12.
[2] SCHOSSERER M,GRUBECK-LOEBENSTEIN B,GRILLARI J.Principles of biological aging[J].Z Gerontol Geriatr,2015,48(3):285-294.
[3] QUIRS P M,LANGER T,LPEZ-OTN C.New roles for mitochondrial proteases in health,ageing and disease[J].Nat Rev Mol Cell Biol,2015,16(6):345-359.
[4] CHILDS B G,BAKER D J,KIRKLAND J L,et al.Senescence and apoptosis:dueling or complementary cell fates?[J].EMBO Rep,2014,15(11):1139-1153.
[5] MOMBACH J C,BUGS C A,CHAOUIYA C.Modelling the onset of senescence at the G1/S cell cycle checkpoint[J].BMC Genomics,2014,15(Suppl 7):S7.
[6] BALDUCCI L,BLASI A,SALDARELLI M,et al.Immortalization of human adipose-derived stromal cells:production of cell lines with high growth rate,mesenchymal marker expression and capability to secrete high levels of angiogenic factors[J].Stem Cell Res Ther,2014,5(3):63.
[7] 庄金秋,梅建国,王文秀,等.细胞永生化技术及其应用研究进展[J].生命科学研究,2011,15(4):363-368.
[8] WANG L,XIAO H,ZHANG X,et al.The role of telomeres and telomerase in hematologic malignancies and hematopoietic stem cell transplantation[J].J Hematol Oncol,2014,20(7):61.
[9] KARLSSON J,LILLJEBJRN H,HOLMQUIST-MENGELBIER L,et al.Activation of human telomerase reverse transcriptase through gene fusion in clear cell sarcoma of the kidney[J].Cancer Lett,2015,357(2):498-501.
[10] PARK Y J,KIM E K,MOON S,et al.Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas[J].Anticancer Res,2014,34(11):6389-6395.
[11] 盖维,戴春,吕昌云.骨髓间充质干细胞对糖尿病肾病大鼠肾脏podocin表达的影响[J].现代医学,2014,42(10):1121-1126.
[12] 金光鑫,吴德全.间充质干细胞治疗肝脏疾病的研究进展[J].现代医学,2013,41(9):690-693.
[13] VILLOTA C,CAMPOS A,VIDAURRE S,et al.Expression of mitochondrial non-coding RNAs(ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes[J].J Biol Chem,2012,287(25):21303-21315.
[14] STEPANENKO A A,KAVSAN V M.Immortalization and malignant transformation of eukaryotic cells[J].Tsitol Genet,2012,46(2):36-75.
[15] 汪廷乐,宋萌.人永生化细胞系模型建立的方法[J].上海口腔医学,2010,19(2):212-215.
[16] WANG M L,WALSH R,ROBINSON K L,et al.Gene expression signature of c-MYC-immortalized human fibroblasts reveals loss of growth inhibitory response to TGF-β[J].Cell Cy-cle,2011,10(15):2540-2548.
[17] 孟凡迎.人原代肝细胞的分离、培养及可逆性永生化[J].中华肝脏病杂志,2009,17(5):395-397.
[18] 潘小平.永生化人肝星状细胞系的建立及其对肝细胞功能与肝祖细胞分化的影响.杭州:浙江大学,2013.
[19] 马迪,严佶祺,彭承宏.肝细胞永生化研究进展[J].组织工程与重建外科杂志,2012,8(1):46-48.
[20] PAN X,WANG Y,YU X,et al.Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes[J].Int J Med Sci,2015,12(3):248-255.
[21] 李爱民,王亚东,王泽楠,等.无病毒可回复性肝细胞永生化载体的构建和结构鉴定[J].现代消化及介入诊疗,2012,17(2):61-65.
[22] SIDDIQI S,SUSSMAN M A.The heart:mostly postmitotic or mostly premitotic? Myocyte cell cycle,senescence,and quiescence[J].Can J Cardiol,2014,30(11):1270-1278.
[23] DONG F,HUANG Y,LI W,et al.The isolation and characterization of a telomerase immortalized goat trophoblast cell line[J].Placenta,2013,34(12):1243-1250.
[24] OMI H,OKAMOTO A,NIKAIDO T,et al.Establishment of an immortalized human extravillous trophoblast cell line by retroviral infection of E6/E7/hTERT and its transcriptional profile during hypoxia and reoxygenation[J].Int J Mol Med,2009,23(2):229-236.
[25] ZHANG L,ZHANG W,SHAO C,et al.Establishment and characterization of a spontaneously immortalized trophoblast cell line(HPT-8) and its hepatitis B virus-expressing clone[J].Hum Reprod,2011,26(8):2146-2156.