Objective: To study the effect of hOGG1 on human retinal pigment epithelial cells in H2O2 induced oxidative stress, by observing pAd-CMV5-DEST-hogg1 transfecting APRE-19. Methods: Construct recombinant adenovirus pAd-CMV5-DEST-hogg1, control adenovirus vector pAd/CMV/V5-GW/lacZ, and transfect them into ARPE-19 cells(RPE-Ad-hogg1, RPE-Ad-lacZ) respectively. The two cell lines,together with a normal ARPE-19 cell strain, were treated by H2O2(100 μmol·L-1,2 h). The oxygen free radicals (ROS) production was detected by Flow Cytometry(FCM). Immunohistochemisty assessed 8-oxoguanine(8-oxodG),which is the oxidative damage end products. Results: The construction and packaging of recombinant adenovirus-hogg1 vector were successful, and it could be expressed in ARPE-19 cells. ARPE-19 cells with high hOGG1 expression had a lower ROS and 8-oxodG production(P<0.05) and lower degree of oxidational damage on 8-oxodG.Conclusion: A high expression of hOGG1 in ARPE-19 cells can reduce the degree of oxidative stress induced by H2O2. |
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