两种不同来源血小板进行基于光子晶体技术的血小板抗体检测的对比研究-东南大学学报医学版

两种不同来源血小板进行基于光子晶体技术的血小板抗体检测的对比研究

单位：1. 东南大学附属中大医院血液科, 江苏南京 210009;
2. 苏州大学附属第三医院, 常州市第一人民医院, 江苏常州 313000

分类号：R446

出版年·卷·期（页码）：2015·34·第五期（758-760）

摘要：

目的:比较分别由浓缩血小板、新鲜血液分离血小板所得血小板裂解液,行光子晶体技术检测血小板抗体的效率。方法:检测样本为原发免疫性血小板减少症(ITP)患者32例和正常健康人对照31例。对来源于浓缩血小板和新鲜血液分离的血小板同时进行基于光子晶体技术的血小板特异性抗体检测及单克隆抗体特异性血小板抗原固定术(MAIPA)检测,以MAIPA检测结果作为光子晶体技术检测结果的参照。结果:两组不同来源的血小板行光子晶体的血小板抗体检测敏感性均高于MAIPA,且浓缩血小板组更高。新鲜血液分离组特异性高于MAIPA,浓缩血小板组低于MAIPA。结论:使用新鲜血液分离采集血小板行光子晶体技术检测血小板特异性抗体效率较高。

Objective: To compare the efficiencies of detections of platelet antibodies based on photonic crystal technology of platelets lysate solution obtained from concentrated platelet sample and platelets separated from fresh blood. Methods: The samples for detection came from 32 primary immune thrombocytopenia(ITP) patients and 31 healthy subjects as the control group. MAIPA was used to examine all samples,and the result was used as a reference for the results of photonic crystal detection. Result: The sensitivity of the two different sources of platelet to the detection based on photonic crystals were both higher than MAIPA, especially for the platelet concentration group.The specificity of freshly isolated blood group was higher than MAIPA, and that of the platelet concentration group was lower than MAIPA. Conclusion: It is most efficient for the specificity antibodies in platelets separated from fresh blood to be examined with photonic crystal-based technology.

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