神经激肽-1受体在GERD模型豚鼠咳嗽反射敏感性中的作用-东南大学学报医学版

神经激肽-1受体在GERD模型豚鼠咳嗽反射敏感性中的作用

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学生理药理学系, 江苏南京 210009;
3. 东南大学附属胸科医院呼吸内科, 江苏南京 210029;
4. 广州医科大学附属第一医院, 广东广州 510000

分类号：R-33

出版年·卷·期（页码）：2015·34·第五期（731-735）

摘要：

目的:探究胃食管反流性豚鼠模型肺组织神经激肽-1受体(NK-1R)与咳嗽反射敏感性(CRS)之间的关系。方法:分组建模后评估豚鼠CRS,免疫组化染色检测肺组织NK-1R的表达。结果:模型组CRS增加,肺内NK-1R表达增加(108.48±25.71 vs 38.54±17.62,P<0.05)。肉毒毒素-A注射后较模型组咳嗽次数减少(23.33±7.53 vs 51.17±10.19,P<0.05),NK-1R表达减少(21.59±13.45 vs 108.48±25.71,P<0.05),潜伏时间未见改变(11.76±3.29 vs 8.24±4.73,P>0.05)。结论:胃食管反流性豚鼠气道CRS增高可能与肺组织中NK-1R表达增加有关。

Objective: To explore the relationships between cough reflex sensitivity and the neurokinin-1 receptor expression in lung in the gastroesophageal reflux model. Methods: We built the model and measured cough reflex sensitivity, observed neurokinin-1 receptor in lung by immunohistochemical staining. Results: The cough reflex sensitivity and the numbers of neurokinin-1 receptor-immunoreactive substances increased(108.48±25.71 vs 38.54±17.62, P<0.05)in the model group. Injected botulinum toxin-A into airway, the cough times dropped(23.33±7.53 vs 51.17±10.19, P<0.05), and the number of neurokinin-1 receptor also lowered(21.59±13.45 vs 108.48±25.71, P<0.05),but the cough latency was not significantly different(11.76±3.29 vs 8.24±4.73, P>0.05) compared to the model group. Conclusion: GERD increases the cough reflex sensitivity, and neurokinin-1 receptor in lung may play an important role in it.

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