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FABP4对人主动脉内皮细胞分泌MMP2的影响
作者:胡晓娟  伍婷  王晨曦  高维强  李军 
单位:上海交通大学医学院附属仁济医院 Med-X临床干细胞研究中心, 上海 200127
关键词:脂肪酸结合蛋白4 人主动脉内皮细胞 血小板-内皮细胞黏附分子 基质金属蛋白酶2 
分类号:R602
出版年·卷·期(页码):2015·34·第五期(726-730)
摘要:

目的:探究脂肪酸结合蛋白4(FABP4)对人主动脉内皮细胞分泌基质金属蛋白酶2(MMP2)的影响。方法:体外培养的人主动脉内皮细胞分别用转化生长因子-β(TGF-β)及TGF-β加FABP4抑制剂HTS01037处理,检测处理前后内皮标志物CD31、VE-钙黏蛋白(VE-Cad)的mRNA表达,以及FABP4、MMP2的mRNA表达。结果:人主动脉内皮细胞用TGF-β处理后,内皮标志物CD31与VE-Cadherin mRNA表达升高,FABP4与MMP2的mRNA表达也升高(均P<0.05);用TGF-β加 HTS01037处理后,与单用TGF-β处理相比,CD31、VE-Cad及FABP4、MMP2的表达均降低(均P<0.05)。结论:FABP4与TGF-β促进内皮细胞分泌MMP2的病理过程正相关。

Objective: To investigate the roles of fatty acid binding protein 4(FABP4) in the process of human aortic endothelial cells secreting matrix metalloproteinase-2(MMP2). Methods: Human aortic endothelial cells were treated with TGF-β alone and TGF-β+FABP4 inhibitor HTS01037 respectively, and the mRNA level of CD31, VE-Cadherin, FABP4 and MMP2 were detected before and after the treatments. Results:When treated with TGF-β alone,human aortic endothelial cells express higher level of CD31 and VE-cadherin, as well as FABP4 and MMP2(all P<0.05).When treated with TGF-β together with FABP4 inhibitor HTS01037, the human aortic endothelial cells express lower CD31,VE-Cadherin,FABP4 and MMP2(all P<0.05). Conclusion: FABP4 has a positive correlation with the process of endothelial cells secreting MMP2 promoted by TGF-β.

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