let-7e嵌合小鼠模型的建立和表型分析-东南大学学报医学版

let-7e嵌合小鼠模型的建立和表型分析

单位：苏州大学生物医学研究院, 江苏苏州 215123

分类号：R392.9;R-33

出版年·卷·期（页码）：2015·34·第五期（696-703）

摘要：

目的:探讨微小RNA let-7e在淋巴细胞分化发育中的作用。方法:构建let-7e过表达嵌合小鼠模型,研究let-7e对淋巴组织中T细胞、B细胞、树突状细胞(DC)、髓系来源的抑制性细胞(MDSC)发育的影响;通过流式细胞仪检测MDSC的细胞增殖和细胞凋亡,初步探讨let-7e促使MDSC增多的机制。结果:嵌合小鼠模型检测结果显示:过表达let-7e后B细胞减少,浆细胞样DC(pDC)、经典DC(cDC)增多,而T细胞及亚群比例没有变化;同时,骨髓和脾脏中的MDSC比例升高,其凋亡水平明显降低,但其增殖没有改变。结论:let-7e参与了淋巴细胞的分化和发育;let-7e可以通过抑制细胞凋亡促进MDSC的增多。

Objective: To investigate the function of let-7e on the development of immunocytes. Methods: To study the function of let-7e on the development of immunocytes, the let-7e over-expression chimeric mouse were firstly established by retrovirus infection. Thereafter, the proliferation and apoptosis were evaluated by FACS. Results: Chimeric mouse model was successfully established and confirmed by FACS and qRT-PCR.FACS analysis showed that over-expressed let-7e decreased the percentage of B220⁺ cells both in BM and spleen cells, increased the percentages of pDCs, cDCs,and MDSCs, but did not change the percentage of T cells and its subsets. Further experiments suggested that over-expressed let-7e increased the percentages of MDSCs by inhibiting the apoptosis of MDSCs, but did not alter their proliferation. Conclusion: let-7e is involved in lymphocyte differentiation and development; let-7e over-expression can increase the number of MDSCs by inhibiting apoptosis.

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