Fogarty球囊构建兔颈总动脉损伤及移植内皮祖细胞治疗的动物模型-东南大学学报医学版

Fogarty球囊构建兔颈总动脉损伤及移植内皮祖细胞治疗的动物模型

单位：东南大学附属中大医院介入与血管外科, 江苏省分子影像与功能影像重点实验室, 江苏南京 210009

分类号：R-332;R654.3;R543.4

出版年·卷·期（页码）：2015·34·第四期（582-587）

摘要：

目的:利用Fogarty球囊建立兔颈总动脉球囊损伤及损伤处移植内皮祖细胞(endothelial progenitor cell,EPCs)治疗的动物模型。方法:培养兔外周血中EPCs并以Fe₂O₃磁性纳米颗粒标记。将实验兔随机分为细胞组(12只)和空白组(10只),经股动脉入路利用Fogarty球囊损伤两组兔右侧颈总动脉,并用Fogarty球囊阻断血流后将标记EPCs移植至细胞组血管损伤段,将生理盐水注入空白组;术后7d取2只细胞组兔损伤血管行普鲁士蓝染色;术后3d,1、4、8周行MR扫描;术后8周取损伤血管,行HE、弹力纤维及PCNA染色。结果:术后7d普鲁士蓝染色示损伤内膜可见含铁EPCs附着,MR扫描示术后4周空白组兔损伤血管壁稍有增厚,术后8周明显增厚,细胞组术后4周损伤血管管壁未见明显增厚,8周后稍有增厚。病理学检查示,术后8周细胞组与空白组内膜/中膜比(N/M)分别为0.34±0.05和1.14±0.10(P<0.01),PCNA阳性细胞指数为0.08±0.01和0.31±0.01(P<0.05)。结论:Fogarty球囊成功构建兔颈总动脉损伤狭窄模型并阻断血流实现EPCs定向移植至血管损伤处,参与内膜的修复。

Objective: To establish rabbit carotid artery balloon injury model with transplanting endothelial progenitor cells (EPCs) to the injury region by the Fogarty balloon. Methods: EPCs were isolated from rabbit peripheral blood and were incubated with Fe₂O₃-arginine. Rabbits were randomly divided into cell group(n=12) and blank group(n=10). The Fogarty balloon catheter was induced into the right common carotid artery(CCA) through the right femoral artery, and then blocking blood flow to transplant EPCs and equal amount of normal saline to the balloon injury local via Fogarty balloon in the cell and blank group respectively. Two rabbits in cell group were killed and the injured vessels for Prussian blue staining on 7rd day. MR was performed on 3rd day, 1th, 4th and 8th week after transplantation. The injured vessels for HE, elastic tissue and PCNA staining on 8th week after injury. Results: Prussian blue staining showed iron-labeled cells distributed in the injured endothelium. In blank group, MR showed the injured arterial wall slightly thickened at 4th week after injury, and more obvious with severe stenosis in the lumen at 8th week; In cell group, the injured arterial wall barely thickened at 4th week and slightly thickened at 8th week. The pathological study showed, the neointimal/media ratio(N/M) in cell group and blank group was 0.34±0.05 and 1.14±0.10, respectively(P<0.01); the PCNA positive cell index in cell group and blank group was 0.08±0.01 and 0.31±0.01, respectively(P<0.05), on 8th week after injury. Conclusion: The Fogarty balloon can be used to establish rabbit carotid artery balloon injury model, followed by local transplantation EPCs to the injury region.

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