Objective: To explore the mechanisms of diabetic vascular calcification by studying the indicators of metabolism and vascular calcification in the high-sugar, high-fat diet low density lipoprotein receptor gene deficient (LDLR-/-) mice. Methods: High-sugar, high-fat diet fed LDLR-/- mice was the experimental group (HF group) and conventional diet fed LDLR-/- mice was the control group (CON group). The two groups were fed for 16 weeks. At the end of the experiment, fasting blood glucose (FBS), total cholesterol (CHO), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), fasting insulin (FIns) were detected. Serum advanced glycation end product-peptide (AGE-P) were detected by flow injection analysis. The expressions of MSX2, OPN, Cbfα-1, α-SMA mRNA were analyzed by Real-time quantitative PCR. Aortic atherosclerotic lesions were observed by hematoxylin-eosin staining. Aortic calcified lesions were observed by Von Kossa staining. Results: Compared with CON group,the serum FBS, FIns, HOMA-IR, CHO, TG, LDL, HDL, AGE-P were significantly higher in HF group. The gene expressions of MSX2, OPN, Cbfα-1 increased, while α-SMA decreased. Aortic atherosclerotic lesions were seen in HF group. HF group demonstrated aortic calcification by Von Kossa staining. No aortic calcification was showed in the CON group. Conclusion: The high sugar, high fat diet can increase the metabolism disorder of glucose and lipid in the LDLR-/- mice, and then accelerate aortic calcification. This animal model can be used to study the mechanisms of vascular complications associated with diabetes. |
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