大鼠不同部位脂肪形态及功能的差异-东南大学学报医学版

大鼠不同部位脂肪形态及功能的差异

单位：南京医科大学第一附属医院老年医学科, 江苏南京 210029

分类号：R-332

出版年·卷·期（页码）：2015·34·第三期（399-404）

摘要：

目的:探讨大鼠不同部位脂肪组织形态和功能差异,为更好地解释代谢综合征的发病机制提供依据.方法:收集SD大鼠各部位脂肪组织样本,采用HE染色方法观察脂肪细胞大小和形态,行细胞RNA抽提及cDNA转录,应用实时定量PCR检测脂肪相关基因表达,抽提总蛋白并应用Western blot方法检测脂肪特异性功能蛋白表达.结果:附睾旁、肾周、肠系膜周围等内脏脂肪细胞较皮下脂肪细胞体积大,肩胛间、肩胛下、脊柱旁等棕色脂肪细胞最小.棕色脂肪功能基因(UCP-1、Cidea、PGC-1α)在大鼠的肩胛下、肩胛间、甲状腺下、脊柱旁脂肪组织高度表达,与经典白色脂肪(附睾旁脂肪)相比,差异有统计学意义(P <0.05),而皮下白色脂肪组织(white adipose tissue, WAT)(如腹股沟、腋下、甲状腺旁)及内脏WAT(如附睾旁、肾周、肠系膜周围)则以白色脂肪功能基因(AP2、HOXC9、Leptin)表达为主,与经典棕色脂肪(肩胛下脂肪)比较,差异有统计学意义(P <0.05),且经典皮下WAT(腹股沟皮下)和经典内脏WAT(附睾旁)的棕色、白色脂肪功能基因表达水平差异无统计学意义(P >0.05).附睾旁、肾周、肠系膜周围等内脏脂肪组织炎症因子TNF-α、IL-6、MCP-1表达水平较高,与经典棕色脂肪(肩胛下脂肪)相比差异有统计学意义(P <0.05).结论:不同部位脂肪组织细胞大小以及炎症状态的差异,可能与代谢综合征的发病机制密切相关,而脂肪的棕色功能差异可能与代谢紊乱疾病的发生无明显相关性.

Objective: To explore the difference of adipose forms and functions in rat's different parts in order to provide evidence for better explanation of the pathogenesis of metabolic syndrome. Methods: The adipose tissue samples of rat's different parts were collected. HE staining was adopted to observe the sizes and forms of adipose cells. Cell RNA extraction and cDNA transcription were carried out to accept the inspection with real-time quantified PCR in respect of the expression of related genes. Total protein was extracted and applied with Western blot to inspect the expression of the specific functional protein of fat. Results: The visceral adipose cells around epididymis, kidney, and mesentery were relatively large in size than the subcutaneous adipose cells while the brown adipose cells between scapulars, below scapulars and beside spine were the smallest. The brown adipose functional genes (UCP-1, Cidea, and PGC-1α) were highly expressed in the adipose tissues below scapulars and thyroid as well as beside the spine of rats than the classic white adipose(around epididymis adipose)(P <0.05). The subcutaneous WAT (such as around groin, armpit and thyroid) as well as the visceral WAT (around epididymis, kidney and mesentery) were mainly expressed as white adipose functional genes (AP2, HOXC9 and Leptin) than the classic brown adipose(below scapulars adipose)(P <0.05), and there was no significant difference (P >0.05) in the brown and white adipose functional gene expression of classic subcutaneous(around groin) and visceral(around epididymis) WAT. The inflammatory factors(TNF-α, IL-6 and MCP-1) had high expression level in the visceral adipose tissues around the epididymis, kidney and mesentery than the classic brown adipose(below scapulars adipose)(P <0.05). Conclusion: The difference of the adipose tissue cell size and inflammatory state in different parts of rats may be closely related to the pathogenesis of metabolic syndrome, and the difference in the brown functions of fats may has no significant correlation with the occurrence of metabolic disorders.

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