SF/PLCL纺丝材料复合骨髓间充质干细胞修复兔桡骨骨缺损的实验研究-东南大学学报医学版

SF/PLCL纺丝材料复合骨髓间充质干细胞修复兔桡骨骨缺损的实验研究

作者：郑欣¹ 李东亚² 陈一心¹ 邱旭升¹ 朱彦丞¹ 施鸿飞¹ 王骏飞¹ 熊进¹

单位：1. 南京大学医学院附属鼓楼医院骨科, 江苏南京 210008;
2. 徐州医学院附属医院骨科, 江苏徐州 221000

分类号：R683

出版年·卷·期（页码）：2015·34·第三期（376-380）

摘要：

目的:研究SF/PLCL纺丝材料复合骨髓间充质干细胞修复兔桡骨骨缺损的能力.方法:取兔骨髓间充质干细胞(BMSCs)并制成BMSCs/藻酸钙水凝胶.选择8只6月龄雄性新西兰兔,于双侧桡骨中段制备15 mm大段骨缺损模型.其中实验组双侧植入SF/PLCL后注入BMSCs/藻酸钙水凝胶;对照组单纯行双侧桡骨缺损手术.所有兔于术后12周行CT检查观察骨缺损修复情况,之后处死兔,大体及组织学观察骨修复情况.结果:术后12周CT及组织学检查显示:实验组纺丝材料塌陷,材料部分降解,纤维细胞长入材料内部;骨缺损断端有骨组织沿套管材料生长,但骨缺损未完成桥接.对照组缺损区域软组织填充,骨缺损断端封闭.结论:SF/PLCL套管材料虽具有良好的生物相容性,但尚不足以用于修复兔桡骨骨缺损,其成骨能力及降解能力有待进一步提升.

Objective: To study the osteogenic potential of SF/PLCL electrospinning material composited with bone mesenchymal stem cells (BMSCs) in repairing the radius defection in rabbits. Methods: BMSCs were obained from rabbits and then mixed for BMSCs/Alginate hydrogel. Eight 6-month-old male New Zealand white rabbits were recruited and 15 mm defect was created in both sides of the radius. In the experimental group, SF/PLCL spinning composited with BMSCs/alginate hydrogel was implanted into the bone defect. In the control group, only bilateral surgery of radial defect was performed. Twelve weeks postoperatively, CT scan reconstruction were used to evaluate the bone formation. Then the rabbits were sacrificed and the specimens were harvested for histologic study. The specimens were embedded in paraffin, followed by decalcification and then stained by hematoxylin and eosin (HE) for histomorphometric analysis to evaluate the bone repair. Results:Twelve weeks postoperatively, CT scan reconstruction and HE staining showed that SF/PLCL spinning degraded partly in the experimental group, and the bone formed along the casing stump, but the bone defect was not fully bridged; While in the control group, the defect was filled with soft tissue, and the ends of bone defects were closed. Conclusion: SF/PLCL casing material in this study shows good biocompatibility, but its osteogenic ability and degradation need to be further refined.

参考文献：

[1] 张晓峰,蒋赞利,吕兰欣,等.PHBV 复合 HA 纳米纤维材料修复兔桡骨缺损的实验研究[J].东南大学学报:医学版,2011,30(3):407-413.
[2] 许振,孟洁,张宇,等.磁性纳米纤维复合材料原位诱导体内成骨的研究[J].东南大学学报:医学版,2011,30(1):1-6.
[3] 张明,吴小涛,董寅生.经表面修饰的 PLLA 多孔材料修复骨缺损的实验研究[J].现代医学,2010,38(3):242-245.
[4] 李东亚,郑欣,邱旭升,等.新西兰兔桡骨骨缺损动物模型的制作[J].中华实验外科杂志,2013,30(9):2012.
[5] LI D,XIA Y.Electrospinning of nanofibers:reinventing the wheel[J].Adv Mater,2004,16(14):1151-1170.
[6] HUANG Z M,ZHANG Y Z,KOTAKI M,et al.A review on polymer nanofibers by electrospinning and their applications in nanocomposites[J].Compos Sci Technol,2003,63(15):2223-2253.
[7] 魏延云,王建广,黄磊,等.复合神经生长因子的纳米纤维导管促神经再生的初步研究[J].中华创伤骨科杂志,2009,11(1):51-55.
[8] MA K,CHAN C K,LIAO S,et al.Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells[J].Biomaterials,2008,29(13):2096-2103.
[9] LEE J,TAE G,KIM Y H,et al.The effect of gelatin incorporation into electrospun poly(L-lactide-co-epsilon-caprolactone) fibers on mechanical properties and cytocompatibility[J].Biomaterials,2008,29(12):1872-1879.
[10] HORAN R L,ANTLE K,COLLETTE A L,et al.In vitro degradation of silk fibroin[J].Biomaterials,2005,26(17):3385-3393.
[11] MURPHY A R,JOHN P S,KAPLAN D L.Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation[J].Biomaterials,2008,29(19):2829-2838.
[12] MEINEL L,HOFMANN S,KARAGEORGIOU V,et al.The inflammatory responses to silk films in vitro and in vivo[J].Biomaterials,2005,26(2):147-155.
[13] KEUN K I,KIDOAKI S,MATSUDA T.Electrospun nano-to microfiber fabrics made of biodegradable copolyesters:structural characteristics,mechanical properties and cell adhesion potential[J].Biomaterials,2005,26(18):3929-3939.
[14] 何晓敏,付炜,王浩,等.利用 Collagen/PLCL 复合纳米纤维电纺膜构建组织工程化气管补片的初步研究[J].中华临床医师杂志:电子版,2012,6(22):7095-7099.
[15] KIM B S,MOONEY D J.Development of biocompatible synthetic extracellular matrices for tissue engineering[J].Trends Biotechnol,1998,16(5):224-230.
[16] 李纲,钱婷婷,洪佳旭,等.丝素蛋白和乳酸-己内酯共聚物纳米纤维支架构建组织工程化角膜上皮的实验研究[J].中国眼耳鼻喉科杂志,2013,13(2):96-99.
[17] 钱卫庆,王宸,陈昌红.BCP/HAFG—rhBMP-2 复合人工骨修复骨缺损的实验研究[J].现代医学,2007,35(4):265-270.
[18] BODDE E W,SPAUWEN P H,MIKOS A G,et al.Closing capacity of segmental radius defects in rabbits[J].J Biomed Mater Res A,2008,85(1):206-217.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录