载阿霉素的磁性微泡双模式的成像效果及其对淋巴瘤细胞毒性的研究-东南大学学报医学版

载阿霉素的磁性微泡双模式的成像效果及其对淋巴瘤细胞毒性的研究

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属中大医院肿瘤科, 江苏南京 210009

分类号：R313;R730.41;R733

出版年·卷·期（页码）：2015·34·第三期（365-368）

摘要：

目的:探讨多功能高分子微泡(MPMBs)双模式成像效能及其联合超声对DLBCL弥漫大B细胞淋巴瘤(DLBCL)细胞株SU-DHL-4增殖和凋亡的影响.方法:以机械振荡法制备共载阿霉素及超顺磁性氧化铁的MPMBs,观察其体外增强B超、磁共振显影的能力;将SU-DHL-4细胞株按处理方法不同分组,CCK-8法检测各组细胞活力,吖啶橙/溴化乙啶(AO/EB)双染法观察细胞形态.结果:MPMBs较普通高分子微泡(PMBs)具有更好的超声、磁共振显影效果;MPMBs联合超声对DLBCL细胞增殖的抑制强于其他处理组.结论:MPMBs是一种可集治疗与超声、磁共振成像于一体的多功能造影剂.

Objective: To investigate the dual-mode imaging ability of multifunctional polymer microbubbles(MPMBs), and to observe its effect on cell proliferation and apoptosis in SU-DHL-4 cells.Methods: The MPMBs were made by mechanical vibration method. Enhancement of ultrasound (US)/magnetic resonance(MR) imaging were evaluated in vitro. The SU-DHL-4 cells were divided into several groups according to different treatments, then the viability of cells were measured by CCK-8 assay and the morphologic changes were detected by AO/EB staining.Results: US/MR dual-mode imaging showed its better property compared with ordinary polymer microbubbles(PMBs). Besides, anti-proliferation effect of MPMBs combined US on cells was much better than other treatment groups. Conclusion: The MPMBs can be served as a mutifunctional imaging agent for treatment and imaging.

参考文献：

[1] SCHINASI L,LEON M E.Non-Hodgkin lymphoma and occupational exposure to agricultural pesticide chemical groups and active ingredients:a systematic review and meta-analysis[J].Int J Environ Res Public Health,2014,11(4):4449-4527.
[2] SIEGEL R,MA J,ZOU Z,et al.Cancer statistics,2014[J].CA Cancer J Clin,2014,64(1):9-29.
[3] MORTON L M,WANG S S,DEVESA S S,et al.Lymphoma incidence patterns by WHO subtype in the United States,1992-2001[J].Blood,2006,107(1):265-276.
[4] National Comprehensive Cancer Network.NCCN Clinical Practice Guidelines in Oncology:Non-Hodgkin lymphoma(version 5.2014)[EB/OL].Fort Washington:NCCN,2014[2014-10-28].http://www.nccn.org/professionals/physician_gls/f_guidelines.asp.
[5] YANG K,FENG L,SHI X,et al.Nano-graphene in biomedicine:theranostic applications[J].Chem Soc Rev,2013,42(2):530-547.
[6] 王庆捷,任利群,童嘉毅,等.超声微泡在心血管疾病治疗领域应用的研究进展[J].现代医学,2011,39(2):248-251.
[7] YANAQISAWA K,MORIYASU F,MIYAHARA T,et al.Phagocytoisis of ultrasound agent microbubbles by Kupffercells[J].Ultrasound Med Boil,2007,33(2):318-325.
[8] RUAN J,HAJJAR K,RAFII S,et al.Angiogenesis and antiangiogenic therapy in non-Hodgkin's lymphoma[J].Ann Oncol,2009,20(3):413-424.
[9] KOPIC E,CICKUSIC E,KOPIC A,et al.Morphometric angiogenesis parameters for indolent and aggressive non-Hodgkin's lymphoma[J].Med Arh,2011,65(1):9-12.
[10] STEIDL C,LEE T,SHAH S P,et al.Tumor-associated macrophages and survival in classic Hodgkin's lymphoma[J].N Engl J Med,2010,362(10):875-885.
[11] WADA N,ZAKI MA,HORI Y,et al.Tumour-associated macrophages in diffuse large B-cell lymphoma:a study of the Osaka Lymphoma Study Group[J].Histopathology,2012,60(2):313-319.
[12] WALTON C B,SHOHET R V.Tiny bubbles and endocytosis？[J].Circ Res,2009,104(5):563-565.
[13] MEIJERING B D,JUFFERMANS L J,Van WAMEL A,et al.Ultra-sound and microbubble-targeted delivery of macromolecules is regulated by induction of endocytosis and pore formation[J].Circ Res,2009,104(5):679-687.

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