>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息：
电磁脉冲诱导大鼠左心室肌组织基因表达谱的变化
作者：李宝丰1  任东青2  郭国祯2  陈蓓3  章莹1  夏虹1 
单位：1. 广州军区广州总医院 骨科, 广东 广州 510010;
2. 第四军医大学 放射医学教研室, 陕西 西安 710032;
3. 广州军区广州总医院 肿瘤科, 广东 广州 510010
关键词：电磁脉冲 大鼠 心肌 基因芯片 表达 
分类号：R82
出版年·卷·期（页码）：2015·34·第二期（171-176）
摘要：

目的:用基因芯片技术研究电磁脉冲(electromagnetic pulse,EMP)辐照对大鼠左心室肌组织的生物效应.方法:10只雄性同窝SD大鼠随机分为辐照组与正常对照组2组,每组5只.在锥形平板GTEM小室内对辐照组大鼠进行EMP辐照,EMP辐照参数为:场强200 kV·m-1,脉冲前沿3.5 ns,脉宽14 ns,重复频率1 Hz;正常对照组进行假辐照.辐照后18 h取大鼠左心室肌,提取RNA,经反转录后用Cy3、Cy5荧光标记,获得两组动物来源cDNA探针,cDNA探针与基因表达谱芯片进行杂交,结果由扫描仪扫描并用软件进行分析统计.结果:筛选出差异表达基因108条,表达增强的有51条,其中功能已知或部分已知的11条,占22%;表达减弱的有57条,其中功能已知或部分已知的15条,占26%.差异表达基因主要涉及代谢、神经递质、肌肉收缩、凋亡、应激等方面.其中蛋白激酶C、热休克蛋白70、辅酶Q10、亚甲基四氢叶酸脱氢酶、5-羟色胺受体2C、锌指蛋白10、干扰素γ、雄激素调节蛋白、雌激素调节蛋白等可能在EMP对大鼠心脏生物学效应方面起重要作用.结论:EMP辐照可诱导大鼠左心室肌组织多个基因特异性表达.

Objective: To explore the bio-effects of electromagnetic pulse(EMP) on left ventricle myocardium of rats by the means of gene chip. Methods: 10 male SD rats were randomly divided into two groups with 5 for each group. One group was subjected to EMP fields(200 kV·m-1, 3.5 ns duration, 14 ns width, 1 Hz) and the other as control. 18 hours after the irradiation, the rats were sacrificed and their left ventricle myocardium were eviscerated. The fluorescent cDNA probes labeled with Cy3 and Cy5 were prepared from RNA extracted from the ventricle myocardium of the two groups. Probes of the two groups were then hybridized against cDNA gene chip, the fluorescent signals were scanned with a scanner and the results were analyzed by computer. Results: Compared with the control, 108 genes in gene expression profile were altered. The expression levels of 51 genes were up-regulated distinctly while 57 genes were down-regulated significantly involved in metabolism, neurotransmitter, muscle contraction, apoptosis, stress response and so on. Among the 108 genes, PKC, HSP70, ubiquinone oxidoreductase, methylene tetrahydrofolate dehydrogenase, 5-hydroxytryptamine receptor 2C, zinc finger protein 10, interferon gamma, androgen regulated 20 kDa protein, estrogen receptor associated protein and so on might have important roles on the cardiac biological effects by EMP. Conclusion: EMP exposure in this study can induce specific expressions of some genes in left ventricle myocardium of rats.
参考文献：

[1] 李丹明,任东青,武新虎,等.电磁辐射对清醒大鼠动脉收缩压的影响及其机制研究[J].中国医学物理学杂志,2007,24(5):352-355.
[2] LI B F,GUO G Z,REN D Q,et al.Electromagnetic pulses induce fluctuations in blood pressure in rats[J].Int J Radiat Biol,2007,83(6):425-429.
[3] 李晓娟,任东青,周咏春,等.电磁脉冲辐照对大鼠心肌闰盘形态及ZO-1、Cx43蛋白表达的影响[J].心脏杂志,2008,20(4):389-393.
[4] SCHENA M,SHALON D,DAVIS R W et al.Quantitative monitoring of gene expression patterns with a complementary DNA microarray[J].Science,1995,270(35):467-470.
[5] STOLC V,SAMANTA M P,TONGPRASIT W,et al.Identification of transcribed sequences in Arabidopsis thaliana by using high-resolution genome tiling arrays[J].Pro Natl Acad Sci U S A,2005,102(12):4453-4458.
[6] 汤永辉,石欣,卫文俊.利用基因芯片技术检测BRAF基因在胰腺癌组织中的表达[J].东南大学学报:医学版,2006,25(5):345-348.
[7] 任东青,金娟,李晓娟,等.电磁脉冲诱导小鼠肠组织基因表达谱的变化[J].卫生研究,2008,37(1):22-24.
[8] KRUSE J J,VELDS A,KERKHOVEN R M,et al.Identification of differentially expressed genes in mouse kidney after irradiation using microarray analysis[J].Radiat Res, 2004,161(1):28-38.
[9] POLI A,MONGIORGI S,COCCO L,et al.Protein kinase C involvement in cell cycle modulation[J].Biochem Soc Trans,2014,42(5):1471-1476.
[10] BUELNA-CHONTAL M,GUEVARA-CHAVEZ J G,SILVA-PALACIOS A,et al.Nrf2-regulated antioxidant response is activated by protein kinase C in postconditioned rat hearts[J].Free Radic Biol Med,2014,74:145-156.
[11] CAPOGROSSI M C,KAKU T,FILBURN C R,et al.Phorbolester and dioctanoylglycerol stimulate membrane association of protein kinase C and have a negative inotropic effect mediated by changes in cytosolic Ca2+ in adult rat cardiac myocytes[J].Circ Res,1990,66(4):1143-1155.
[12] 傅聪,姚玉宇.激肽释放酶-激肽系统在缺血损伤后血管新生中的作用研究进展[J].东南大学学报:医学版,2012,31(6):756-760.
[13] 黄长根,姚玉宇,马根山.激肽释放酶 - 激肽系统对心梗后心肌细胞灌注的影响[J].东南大学学报:医学版,2013,32(5):626-630.
[14] LI Z,SRIVASTAVA P.Heat-shock proteins[J].Curr Protoc Immunol,2004,10(2):735-743.
[15] 崔凯,张军,李宝丰,等.电磁脉冲照射前后大鼠心肌超声背向散射参数变化的研究[J].心脏杂志,2006,18(3):293-296.
[16] FERREIRA H S,OLIEVIRA E,FAUSTINO T N et al.Effect of the activation of central 5-HT2C receptors by the 5-HT2C agonist mCPP on blood pressure and heart rate in rats[J].Brain Research,2005,1040(1-2):64-72.
[17] HECKER J G,McGARVEY M.Heat shock proteins as biomarkers for the rapid detection of brain and spinal cord ischemia:a review and comparison to other methods of detection in thoracic aneurysm repair[J].Cell Stress Chaperones,2011,16(2):119-131.
[18] 王晋,郎海洋,张杰,等.脉冲电磁场对大鼠星形胶质细胞和C6细胞热休克蛋白表达的影响[J].生物物理学报,2009,25(增刊):473-475.
[19] YANG H,YUAN W,WANG Y,et al.ZNF649,a novel Kruppel type zinc-finger protein,functions as a transcriptional suppressor[J].Biochem Biophys Res Commun,2005,333(1):206-215.
[20] FERREIRA L R,FRADE A F,BARON M A,et al.Interferon-γ and other inflammatory mediators in cardiomyocyte signaling during Chagas disease cardiomyopathy[J].World J Cardiol,2014,6(8):782-790.
服务与反馈：
【文章下载】【发表评论】【查看评论】【加入收藏】
提示：您还未登录，请登录！点此登录
您是第 415945 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话：025-83272481 83272483
电子邮件： bjb@pub.seu.edu.cn

苏ICP备09058364