核素标记IL-11分子探针的构建及MHCC97H肝癌显像的研究-东南大学学报医学版

核素标记IL-11分子探针的构建及MHCC97H肝癌显像的研究

作者：周欢¹ 吴清华² 陈道桢³ 李怡佳¹ 孙晋⁴ 张俊⁴ 王强⁴ 谢彦⁴ 钟英⁴ 刘璐⁴ 周石¹

单位：1. 贵阳医学院附属医院介入科, 贵州贵阳 550004;
2. 无锡市第三人民医院影像中心, 江苏无锡 214041;
3. 南京医科大学附属无锡妇幼保健院, 江苏无锡 214002;
4. 东南大学附属中大医院核医学科, 江苏南京 210009

关键词：九环肽靶向探针肝癌核素

分类号：R735.7;R817

出版年·卷·期（页码）：2015·34·第一期（32-36）

摘要：

目的:构建核素标记自主合成九环肽DTPA-c(CGRRAGGSC)的生物分子靶向探针,研究其在肝癌体内分布及细胞定位。方法:Western blot检测人肝癌细胞MHCC97H及人正常肝细胞7702白介素11受体(IL-11R)表达情况;进行标记探针与MHCC97H细胞荧光定位;确定⁹⁹Tc^m标记DTPA-c(CGRRAGGSC)最佳条件;观察标记探针⁹⁹Tc^m-DTPA-c(CGRRAGGSC)在MHCC97H肝癌模型鼠体内生物分布。结果:IL-11R表达量MHCC97H细胞是7702细胞的21倍;荧光实验证实DTPA-c(CGRRAGGSC)与MHCC97H细胞膜和胞浆特异性结合;⁹⁹Tc^m-DTPA-c(CGRRAGGSC)标记率>98%;瘤体给药后进行ECT显像,证实具有良好靶向性及稳定性。结论:核素及荧光标记九环肽可靶向特异性结合肝癌MHCC97H肿瘤模型。

Objective: To built a nuclide biological target probe and assess the biostribution in subcutaneous transplanted model of MHCC97H in nude mice. Methods: The expression changes of IL-11R in MHCC97H tumors and 7702 nomal hepatic cell were examined by Western blot. The binding site of the molecular target and internalization with MHCC97H cells was observed under a fluorescence microscope. DTPA-c(CGRRAGGSC) was radiolabeled with ⁹⁹Tc^m. The biodisrtibution in nude mice bearing MHCC97H tumors after injection of ⁹⁹Tc^m-DTPA-c(CGRRAGGSC) molecular probe was obsored. Results: Western blot showed the expression of IL-11R in MHCC97H cells was 21 times than that of 7702 cells. The c(CGRRAGGSC) possessed the ability of specific binding with cytomembrane of MHCC97H cells.The labeling rate of ⁹⁹Tc^m-DTPA-c(CGRRAGGSC) was more than 98%. ECT imags confirmed good targeting and stability of ⁹⁹Tc^m-DTPA-c(CGRRAGGSC) probe in nude mice bearing MHCC97H. Conclusion: Nuclide marked DTPA-c(CGRRAGGSC) has specific binding to the MHCC97H tumor model of liver cancer

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