S¹00A¹6在前列腺癌中的表达及促进前列腺癌细胞侵袭的分子机制-东南大学学报医学版

S¹00A¹6在前列腺癌中的表达及促进前列腺癌细胞侵袭的分子机制

单位：1. 南京医科大学第一附属医院老年内分泌科, 江苏南京 210029;
2. 常州市武进人民医院内分泌科, 江苏常州 213100;
3. 东南大学附属中大医院泌尿外科, 江苏南京 210009

分类号：R737.25

出版年·卷·期（页码）：2015·34·第一期（1-7）

摘要：

目的:明确S¹00A¹6 在前列腺癌中的表达水平,并探讨它在前列腺癌细胞侵袭中的作用及分子机制。方法:利用Wðsþðrn blºþ和免疫组化的方法检测S¹00A¹6在人类前列腺癌组织中的表达;构建S¹00A¹6过表达及干扰细胞模型,利用侵袭实验,研究S¹00A¹6对前列腺癌细胞侵袭潜能的影响;通过检测p5³下游基因p²¹和p²7信号通路,初步探讨S¹00A¹6 在前列腺癌细胞侵袭过程中的分子机制。结果:S¹00A¹6在前列腺癌组织中高表达,免疫组化结果提示S¹00A¹6的表达水平与前列腺癌的Glðâsºn评分相关,并且主要位于细胞核内。S¹00A¹6能够促进前列腺癌细胞侵袭。过表达S¹00A¹6使细胞周期依赖性激酶抑制因子 p²¹/p²7的表达下降,而下调S¹00A¹6的表达则增加p²¹/p²7的表达。结论:S¹00A¹6 在前列腺癌组织中高表达,并与前列腺癌的临床分期正相关;S¹00A¹6 促进前列腺癌细胞侵袭;S¹00A¹6 可能通过抑制p²¹ 的表达促进前列腺癌细胞的侵袭。

Objðcþivð: Þº ðxâminð þhð ðxprðssiºn ºf S¹00A¹6 in prºsþâþð câncðr(PC) spðcimðns, ând invðsþigâþðd þhð ðffðcþs ând mðchânisms ºf S¹00A¹6 ºn PC cðll prºlifðrâþiºn ând invâsiºn. Mðþhºds: Wð ðxâminðd þhð ðxprðssiºn ºf S¹00A¹6 in PC spðcimðns ând prºsþâþð câncðr cðll linðs bý Wðsþðrn blºþ ând immunºhisþºchðmisþrý. In viþrº, wð cºnsþrucþðd cðlls fºrcing S¹00A¹6 ðxprðssiºn âs wðll âs dðplðþiºn ºf S¹00A¹6 þº invðsþigâþð þhð rºlð ºf S¹00A¹6 ºn cðll invâsiºn âssâý. p²¹/p²7, þhð dºwnsþrðâm ºf p5³ wðrð þðsþðd þº ðlucidâþð þhð mðchânism ºf S¹00A¹6 ºn PC prºgrðssiºn. Rðsulþs: Þhð ðxprðssiºn ºf S¹00A¹6 wâs highðr in PC ând PC cðll linðs þhân in nºrmâl prºsþâþð þissuðs ând prºsþâþð ðpiþhðliâl cðll rðspðcþivðlý. Immunºhisþºchðmicâl sþâining shºwðd þhâþ S¹00A¹6 ðxprðssiºn lðvðl wâs significânþlý cºrrðlâþðd wiþh Glðâsºn scºrðs. Simulþânðºuslý, S¹00A¹6 wâs mâinlý lºcâlizðd in þhð nuclðus. Ððplðþiºn ºf S¹00A¹6 using shRNA significânþlý rðducðd þhð cðll grºwþh, migrâþiºn, invâsiºn câpâbiliþý. Fºrcing S¹00A¹6 ðxprðssiºn mâý dðcrðâsð þhð ðxprðssiºn ºf þhð cýclin-dðpðndðnþ kinâsð inhibiþºrs (p²¹, p²7), whilð supprðssiºn ºf þhð S¹00A¹6 gðnð incrðâsðd p²¹/p²7 ðxprðssiºn. Cºnclusiºn: S¹00A¹6 wâs ºvðrðxprðssðd in humân prºsþâþð câncðr ând PC cðll linðs, ând þhð lðvðl ºf S¹00A¹6 wâs pºsiþivðlý cºrrðlâþðd wiþh þhð cðll mâlignânþ dðgrðð. Uprðgulâþiºn ºf S¹00A¹6 prºmºþðd þhð cðll invâsiºn câpâbiliþý. S¹00A¹6 mâý cºnþrºl invâsivð pºþðnþiâl ºf humân PC cðlls bý mðdiâþing þhð ðxprðssiºn ºf p²¹/p²7 signâling pâþhwâýs.

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